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前病毒插入对小鼠B2m基因转录的影响。

Effect of proviral insertion on transcription of the murine B2mb gene.

作者信息

Frankel W N, Potter T A, Rajan T V

机构信息

Department of Genetics, Albert Einstein College of Medicine, Bronx, New York 10461.

出版信息

J Virol. 1989 Jun;63(6):2623-8. doi: 10.1128/JVI.63.6.2623-2628.1989.

DOI:10.1128/JVI.63.6.2623-2628.1989
PMID:2542575
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC250742/
Abstract

We describe three B2mb mutants caused by integration of retroviral proviruses into the B2m gene. All three insertions occurred within 500 base pairs of each other in the 5' end of the first intron of the B2mb gene. Mutant cell line i1 contained a nonfunctional Abelson murine leukemia virus-initiated transcript that spliced into B2mb exon II; mutant cell lines i7 and i18 contained transcripts complementary to B2mb exon 1. Both i1 and i7 also contained small amounts of wild-type B2mb transcript. The implications of these studies for the development of retroviral insertional mutagenesis as a strategy for cloning previously uncloned genes are discussed.

摘要

我们描述了三种由逆转录病毒前病毒整合到B2m基因中引起的B2mb突变体。所有这三种插入都发生在B2mb基因第一个内含子5'端彼此相距500个碱基对的范围内。突变细胞系i1含有一个无功能的由阿贝尔逊鼠白血病病毒起始的转录本，该转录本剪接到B2mb外显子II中；突变细胞系i7和i18含有与B2mb外显子1互补的转录本。i1和i7还都含有少量野生型B2mb转录本。本文讨论了这些研究对于将逆转录病毒插入诱变作为克隆先前未克隆基因的策略的发展所具有的意义。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11be/250742/291c4b37d0cc/jvirol00073-0225-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11be/250742/86c11f98d816/jvirol00073-0223-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11be/250742/9f711c1b7eab/jvirol00073-0223-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11be/250742/ea4955cf7eef/jvirol00073-0223-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11be/250742/57cd38aca0ce/jvirol00073-0224-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11be/250742/291c4b37d0cc/jvirol00073-0225-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11be/250742/86c11f98d816/jvirol00073-0223-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11be/250742/9f711c1b7eab/jvirol00073-0223-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11be/250742/ea4955cf7eef/jvirol00073-0223-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11be/250742/57cd38aca0ce/jvirol00073-0224-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11be/250742/291c4b37d0cc/jvirol00073-0225-a.jpg

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1
Effect of proviral insertion on transcription of the murine B2mb gene.前病毒插入对小鼠B2m基因转录的影响。
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2
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引用本文的文献

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Nucleic Acids Res. 1989 Sep 25;17(18):7553-78. doi: 10.1093/nar/17.18.7553.
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Intracisternal A-type particle-mediated activations of cytokine genes in a murine myelomonocytic leukemia: generation of functional cytokine mRNAs by retroviral splicing events.

本文引用的文献

1
Nucleotide sequence of Abelson murine leukemia virus genome: structural similarity of its transforming gene product to other onc gene products with tyrosine-specific kinase activity.阿贝尔逊鼠白血病病毒基因组的核苷酸序列：其转化基因产物与其他具有酪氨酸特异性激酶活性的癌基因产物的结构相似性。
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2
Retroviruses as mutagens: insertion and excision of a nontransforming provirus alter expression of a resident transforming provirus.逆转录病毒作为诱变剂：非转化原病毒的插入和切除会改变常驻转化原病毒的表达。
Cell. 1981 Jul;25(1):23-36. doi: 10.1016/0092-8674(81)90228-2.
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脑池内A 型颗粒介导的小鼠骨髓单核细胞白血病中细胞因子基因的激活：通过逆转录病毒剪接事件产生功能性细胞因子mRNA
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4
Structure and expression of a gene encoding a putative GTP-binding protein identified by provirus integration in a transgenic mouse strain.通过转基因小鼠品系中的前病毒整合鉴定出的一种假定GTP结合蛋白编码基因的结构与表达
Mol Cell Biol. 1991 Feb;11(2):886-93. doi: 10.1128/mcb.11.2.886-893.1991.
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
从含有噬菌体SP6启动子的质粒中高效体外合成生物活性RNA和RNA杂交探针。
Nucleic Acids Res. 1984 Sep 25;12(18):7035-56. doi: 10.1093/nar/12.18.7035.
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Insertion mutagenesis of embryonal carcinoma cells by retroviruses.
Science. 1985 May 3;228(4699):554-8. doi: 10.1126/science.3838595.
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Genetic organization of the agouti region of the mouse.小鼠刺鼠信号蛋白基因座的遗传结构
Genetics. 1987 Sep;117(1):93-100. doi: 10.1093/genetics/117.1.93.
6
Spontaneous deletion at the B2m locus: evidence for site-specific genetic rearrangement.
J Immunol. 1987 Feb 15;138(4):1270-4.
7
A mutational hot-spot within an intron of the mouse beta 2-microglobulin gene.小鼠β2-微球蛋白基因一个内含子内的突变热点。
EMBO J. 1986 Jan;5(1):103-11. doi: 10.1002/j.1460-2075.1986.tb04183.x.
8
Mitotic recombination in germ cells generated two major histocompatibility complex mutant genes shown to be identical by RNA sequence analysis: Kbm9 and Kbm6.生殖细胞中的有丝分裂重组产生了两个主要组织相容性复合体突变基因，经RNA序列分析显示它们是相同的：Kbm9和Kbm6。
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9
Retroviruses as tools for mammalian development.逆转录病毒作为哺乳动物发育研究的工具
Symp Fundam Cancer Res. 1986;39:59-65.
10
Two modes of c-myb activation in virus-induced mouse myeloid tumors.病毒诱导的小鼠髓系肿瘤中c-myb激活的两种模式。
Mol Cell Biol. 1986 Feb;6(2):380-92. doi: 10.1128/mcb.6.2.380-392.1986.