无龋和患龋个体唾液诱导变形链球菌对金属托槽的差异黏附
Differential adhesion of Streptococcus mutans to metallic brackets induced by saliva from caries-free and caries-active individuals.
作者信息
do Rosário Junior Ademir Franco, Knop Luégya Amorin Henriques, Baboni Fernanda Brasil, Rymovicz Alinne Ulbrich Mores, Tanaka Orlando Motohiro, Rosa Edvaldo Antonio Ribeiro
机构信息
Department of Orthodontics, The Pontifical Catholic University of Paraná, Curitiba, Brazil Xenobiotics Research Unit, The Pontifical Catholic University of Paraná, Curitiba, Brazil.
出版信息
J Investig Clin Dent. 2011 Aug;2(3):197-200. doi: 10.1111/j.2041-1626.2011.00058.x. Epub 2011 Mar 15.
AIM
The purpose of this study was to evaluate the influence of saliva obtained from caries-free and caries-active individuals on the adhesion rates of Streptococcus mutans to metallic brackets.
METHODS
The unstimulated whole saliva of four caries-free (decayed, missing, or filled surfaces = 0) volunteers and four caries-active (decayed, missing, or filled surfaces >12) patients were collected. The saliva samples from each group were mixed and clarified. Acquired pellicles were formed onto 30 metallic edgewise brackets for premolars for each saliva group. The brackets were put in contact with planktonic cells of Streptococcus mutans ATCC 25175. Adhesion rates were assessed by crystal violet retention technique.
RESULTS
A higher streptococcal adhesion pattern (P < 0.00001) was observed on acquired pellicles formed by saliva from caries-active donors.
CONCLUSIONS
The results showed that saliva from caries-active patients tends to increase the mutans adhesion to surfaces, which is a point of concern for orthodontists.
目的
本研究旨在评估无龋个体和患龋个体的唾液对变形链球菌在金属托槽上黏附率的影响。
方法
收集了4名无龋志愿者（龋失补牙面数=0）和4名患龋患者（龋失补牙面数>12）的非刺激性全唾液。将每组的唾液样本混合并澄清。在每个唾液组的30个用于前磨牙的金属直丝托槽上形成获得性薄膜。将托槽与变形链球菌ATCC 25175的浮游细胞接触。通过结晶紫保留技术评估黏附率。
结果
在由患龋供体的唾液形成的获得性薄膜上观察到更高的链球菌黏附模式（P<0.00001）。
结论
结果表明，患龋患者的唾液倾向于增加变形链球菌在表面的黏附，这是正畸医生关注的一个问题。
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