Ahn Sug-Joon, Lim Bum-Soon, Yang Hyeong-Cheol, Chang Young-Il
Dental Research Institute, Department of Orthodontics, College of Dentistry, Seoul National University, Seoul, South Korea.
Angle Orthod. 2005 Jul;75(4):666-71. doi: 10.1043/0003-3219(2005)75[666:QAOTAO]2.0.CO;2.
The aim of this study was to analyze the adhesion of cariogenic streptococci to orthodontic metal brackets in terms of the type of bacterial strains, the incubation time, and saliva coating. Two strains of Streptococcus mutans (S. mutans LM7 and S. mutans OMZ65) and two strains of S. sobrinus (S. sobrinus B13 and S. sobrinus 6715) were used. Twenty metal brackets were incubated with either unstimulated whole saliva or phosphate-buffered saline for two hours. The bacterial adhesion assays were then performed by incubating the tritium-labeled streptococci with saliva-coated or noncoated orthodontic brackets for three, six, or nine hours. The results showed a characteristic binding pattern according to the type of bacterial strains used. S. mutans OMZ65 showed the highest amount of adhesion, whereas S. sobrinus B13 showed the lowest amount of adhesion. Generally, an extended incubation time increased the adhesion of cariogenic streptococci, and the amount of adhesion was the highest after nine hours of incubation. The saliva coating did not significantly influence the adhesion of bacteria. However, this saliva-mediated adhesion differed according to incubation time. The saliva coating tended to gradually decrease the adhesion by the extended incubation time, compared with the noncoated controls. This study indicates that each strain of cariogenic streptococci has a characteristic adhesion pattern and the type of bacterial strain, the incubation time, and saliva influenced the adhesion.
本研究的目的是根据细菌菌株类型、孵育时间和唾液包被情况，分析致龋性链球菌与正畸金属托槽的黏附情况。使用了两株变形链球菌（变形链球菌LM7和变形链球菌OMZ65）和两株远缘链球菌（远缘链球菌B13和远缘链球菌6715）。将20个金属托槽分别与未刺激的全唾液或磷酸盐缓冲盐水孵育两小时。然后通过将氚标记的链球菌与唾液包被或未包被的正畸托槽孵育三小时、六小时或九小时来进行细菌黏附试验。结果显示，根据所用细菌菌株类型呈现出特征性的结合模式。变形链球菌OMZ65的黏附量最高，而远缘链球菌B13的黏附量最低。一般来说，延长孵育时间会增加致龋性链球菌的黏附，孵育九小时后黏附量最高。唾液包被对细菌黏附没有显著影响。然而，这种唾液介导的黏附因孵育时间而异。与未包被的对照组相比，随着孵育时间延长，唾液包被倾向于使黏附逐渐减少。本研究表明，每种致龋性链球菌菌株都有特征性的黏附模式，细菌菌株类型、孵育时间和唾液都会影响黏附。
