在聚集免疫纳米金溶胶基质中使用无标记维多利亚蓝B作为探针的表面增强拉曼光谱法定量检测痕量人绒毛膜促性腺激素
SERS quantitative detection of trace human chorionic gonadotropin using a label-free Victoria blue B as probe in the aggregated immunonanogold sol substrate.
作者信息
Ma Lu, Wen Guiqing, Ye Lingling, Lu Zujun, Luo Yanghe, Liang Aihui, Jiang Zhiliang
机构信息
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Ministry of Education, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guangxi Normal University, Guilin, 541004, China.
出版信息
Luminescence. 2015 Sep;30(6):790-7. doi: 10.1002/bio.2821. Epub 2014 Nov 27.
Nanogold particles (NG) were modified by anti-rabbit antibody (RAb) against human chorionic gonadotropin to obtain an immunonanogold probe (ING). In pH 7.0 Na2HPO4-citrate buffer solution containing KCl, ING probes formed large aggregates in which Victoria blue B (VBB) molecules were adsorbed on the surface and which exhibited strong surface-enhanced Raman scattering (SERS) at a peak of 1612 cm(-1). After addition of human chorionic gonadotropin (hCG) an immune reaction with the ING probe occurred to form dispersive ING-hCG complexes with non-SERS activity that led to a decreased SERS peak at 1612 cm(-1). The decreased SERS intensity was linear to the concentration of hCG over 2.4-73.2 ng/mL. The ING reaction was studied in detail by SERS, scanning electron microscope (SEM), resonance Rayleigh scattering (RRS), surface plasmon resonance (SPR) absorption and laser scattering techniques. SERS quenching was observed and discussed.
用抗人绒毛膜促性腺激素的抗兔抗体（RAb）修饰纳米金颗粒（NG），以获得免疫纳米金探针（ING）。在含有KCl的pH 7.0的磷酸氢二钠 - 柠檬酸盐缓冲溶液中，ING探针形成大聚集体，维多利亚蓝B（VBB）分子吸附在其表面，并且在1612 cm⁻¹的峰处表现出强烈的表面增强拉曼散射（SERS）。加入人绒毛膜促性腺激素（hCG）后，与ING探针发生免疫反应，形成具有非SERS活性的分散性ING - hCG复合物，导致1612 cm⁻¹处的SERS峰降低。SERS强度的降低与2.4 - 73.2 ng/mL范围内的hCG浓度呈线性关系。通过SERS、扫描电子显微镜（SEM）、共振瑞利散射（RRS）、表面等离子体共振（SPR）吸收和激光散射技术详细研究了ING反应。观察并讨论了SERS猝灭现象。
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