Luo Yanghe, Wang Xiaoliang, Liu Qingye, Liang Aihui, He Xingcun, Jiang Zhiliang
School of Food and Bioengineering, Hezhou University, Hezhou, China.
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry Education, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin, China.
Luminescence. 2018 Feb;33(1):131-137. doi: 10.1002/bio.3382. Epub 2017 Aug 11.
Using silver nanoparticles (AgNPs) as the nanocatalyst, l-cysteine rapidly reduced HAuCl to make a stable gold nanoparticle sol (Ag/AuNP) that had a high surface-enhanced Raman scattering (SERS) activity in the presence of Victoria blue 4R (VB4r) molecular probes. Under the selected conditions, chondroitin sulfate (Chs) reacted with the VB4r probes to form associated complexes that caused the SERS effect to decrease to 1618 cm . The decreased SERS intensity was linear to the Chs concentration in the range 3.1-500 ng/ml, with a detection limit of 1.0 ng/ml Chs. Accordingly, we established a simple and sensitive SERS quantitative analysis method to determine Chs in real samples, with a relative standard deviation of 1.47-3.16% and a recovery rate of 97.6-104.2%.
以银纳米颗粒（AgNPs）作为纳米催化剂，L-半胱氨酸迅速将氯金酸还原，制成一种稳定的金纳米颗粒溶胶（Ag/AuNP），该溶胶在维多利亚蓝4R（VB4r）分子探针存在下具有高表面增强拉曼散射（SERS）活性。在选定条件下，硫酸软骨素（Chs）与VB4r探针反应形成缔合络合物，导致SERS效应降低至1618 cm。SERS强度的降低与Chs浓度在3.1 - 500 ng/ml范围内呈线性关系，Chs的检测限为1.0 ng/ml。据此，我们建立了一种简单灵敏的SERS定量分析方法来测定实际样品中的Chs，相对标准偏差为1.47 - 3.16%，回收率为97.6 - 104.2%。
