Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin 541004, China.
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin 541004, China.
Mater Sci Eng C Mater Biol Appl. 2019 Jun;99:1399-1406. doi: 10.1016/j.msec.2019.02.080. Epub 2019 Feb 22.
The highly catalytic Ca-doped carbon dots (CD) were prepared by microwave procedure, that exhibit strong catalytic effect on HAuCl-glucose (GLC) reaction to form gold nanoparticles (AuNPs) with high SERS activity, using Victoria blue B (VBB) as a molecular probe. The SERS intensity at 1615 cm increased linearly with CD increasing, due to formation of more AuNPs nanosol substrate as indicator. When thrombin aptamer (Apt) was added in this system, Apt adsorbed on the CD surface to inhibit theirs catalytic activity, and the SERS intensity decreased. However, when thrombin (TB) was present, it can bind to Apt to form stable G-duplex of Apt-TB and free CD catalyst in the system, and the SERS signal increased linearly. Thus a free-label Apt-SERS quantitative analysis method was developed for ultratrace TB, with a linear range of 0.0058-0.115 nmol/L and a detection limit of 0.0018 nmol/L TB.
采用微波法制备了高催化活性的钙掺杂碳点（CD），其对 HAuCl-葡萄糖（GLC）反应具有很强的催化作用，可形成具有高表面增强拉曼散射（SERS）活性的金纳米粒子（AuNPs），并以维多利亚蓝 B（VBB）作为分子探针。由于形成了更多的 AuNPs 纳米溶胶基质作为指示剂，在 1615 cm 处的 SERS 强度随 CD 的增加呈线性增加。当向该体系中加入凝血酶适体（Apt）时，Apt 吸附在 CD 表面上，抑制其催化活性，SERS 强度降低。然而，当存在凝血酶（TB）时，它可以与 Apt 结合形成 Apt-TB 的稳定 G-二聚体和体系中游离的 CD 催化剂，SERS 信号呈线性增加。因此，建立了一种用于痕量 TB 的无标记 Apt-SERS 定量分析方法，其线性范围为 0.0058-0.115 nmol/L，检测限为 0.0018 nmol/L TB。
