马尾藻对慢性 10 微米煤尘暴露大鼠肺泡巨噬细胞及支气管肺泡灌洗液丙二醛水平的影响。
The effects of Eucheuma cottonii on alveolar macrophages and malondialdehyde levels in bronchoalveolar lavage fluid in chronically particulate matter 10 coal dust-exposed rats.
机构信息
Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia.
Department of Medical Chemistry and Biochemistry, Research Center for Toxicology, Cancer and Degenerative Disease, Medical Faculty, Lambung Mangkurat University, Banjarmasin, South Kalimantan, Indonesia.
出版信息
Iran J Basic Med Sci. 2014 Jul;17(7):541-5.
OBJECTIVES
To investigate the effect of Eucheuma cottonii on alveolar macrophages (AM) and malondialdehyde (MDA) levels in bronchoalveolar lavage fluids (BALF) in particulate matter 10 (PM10) coal dust-exposed rats.
MATERIALS AND METHODS
Ten groups, including a non exposed group and groups exposed to coal dust at doses of 6.25 (CD6.25), 12.5 (CD12.5), or 25 mg/m(3) (CD25) an hour daily for 6 months with or without supplementation of ethanolic extract of E. cottonii at doses of 150 (EC150) or 300 mg/kg BW (EC300). The number of macrophages was determined using a light microscope. MDA levels were measured by TBARS assay.
RESULTS
EC150 insignificantly (P > 0.05) reduces the AM in CD groups compared to non treatment groups. EC150 and EC300 significantly (P < 0.05) decreased MDA levels in CD12.5 and CD25 groups relative to non treatment groups.
CONCLUSION
E. cottonii attenuated oxidative stress in chronic exposure of PM10 coal dust.
目的
研究麒麟菜对 10 微米煤尘暴露大鼠肺泡巨噬细胞（AM）和支气管肺泡灌洗液（BALF）中丙二醛（MDA）水平的影响。
材料和方法
包括非暴露组和暴露于 6.25（CD6.25）、12.5（CD12.5）或 25 毫克/立方米（CD25）煤尘剂量的 10 组，每天 1 小时，持续 6 个月，同时或不补充麒麟菜乙醇提取物剂量为 150（EC150）或 300 毫克/千克体重（EC300）。使用光学显微镜测定巨噬细胞数量。通过 TBARS 测定法测量 MDA 水平。
结果
与非治疗组相比，EC150 对 CD 组的 AM 无显著影响（P>0.05）。EC150 和 EC300 可显著降低（P<0.05）CD12.5 和 CD25 组相对于非治疗组的 MDA 水平。
结论
麒麟菜减轻了 PM10 煤尘慢性暴露引起的氧化应激。
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