Extract and Dieckol Attenuate Cellular Lipid Peroxidation in Keratinocytes Exposed to PM10.

Airborne particulate matter can cause oxidative stress, inflammation, and premature skin aging. Marine plants such as Kjellman contain high amounts of polyphenolic antioxidants. The purpose of this study was to examine the antioxidative effects of extract in cultured keratinocytes exposed to airborne particulate matter with a diameter of <10 m (PM10). After the exposure of cultured HaCaT keratinocytes to PM10 in the absence and presence of extract and its constituents, cell viability and cellular lipid peroxidation were assessed. The effects of eckol and dieckol on cellular lipid peroxidation and cytokine expression were examined in human epidermal keratinocytes exposed to PM10. The total phenolic content of extract was the highest among the 50 marine plant extracts examined. The exposure of HaCaT cells to PM10 decreased cell viability and increased lipid peroxidation. The PM10-induced cellular lipid peroxidation was attenuated by extract and its ethyl acetate fraction. Dieckol more effectively attenuated cellular lipid peroxidation than eckol in both HaCaT cells and human epidermal keratinocytes. Dieckol and eckol attenuated the expression of inflammatory cytokines such as tumor necrosis factor- (TNF-) , interleukin- (IL-) 1, IL-6, and IL-8 in human epidermal keratinocytes stimulated with PM10. This study suggested that the polyphenolic constituents of , such as dieckol, attenuated the oxidative and inflammatory reactions in skin cells exposed to airborne particulate matter.