Ergür Bekir Uğur, Çilaker Mıcılı Serap, Yılmaz Osman, Akokay Pınar
Department of Histology and Embryology, Faculty of Medicine, Dokuz Eylül University; İzmir-Turkey.
Anatol J Cardiol. 2015 Jun;15(6):443-9. doi: 10.5152/akd.2014.5483. Epub 2014 Apr 28.
The present study was designed to investigate the effects of α-lipoic acid on the abdominal aorta and hypertension in a remnant kidney model histomorphometrically, immunohistochemically, and ultrastructurally.
We surgically reduced the renal tissue mass to 5/6 by applying a remnant kidney model. The rats were divided into 4 groups: Group 1- control group, Group 2- lipoic acid group, Group 3- 5/6 nephrectomy group, and Group IV: 5/6 nephrectomy+lipoic acid-treated group. Lipoic acid solution 100 mg/kg was administered by oral gavage for 8 weeks to Groups II and IV. At the end of the experiment, systemic mean blood pressure was monitored. Then, aortic tissues were removed and fixed. After routine histological procedures, tissue sections were examined histochemically, immunohistochemically (type I angiotensin receptor, vascular endothelial growth factor, alpha-smooth muscle actin), and ultrastructurally.
The blood pressure measurements in 5/6 nephrectomy group were significantly higher compared to other groups. In the 5/6 nephrectomy+lipoic acid group, measured blood pressure values and tunica media thickness were significantly lower than in the 5/6 nephrectomy group. In the 5/6 nephrectomy+lipoic acid group, decreased aortic wall thickness, regularity in the structure of elastic fibrils, and more organized elastic lamellae were seen. The expression of type I angiotensin receptor, vascular endothelial growth factor, alpha-smooth muscle actin in the 5/6 nephrectomy+lipoic acid group was decreased compared to the 5/6 nephrectomy group.
In the present study, we found that α-lipoic acid could be a favorable agent for the target organ effects of secondary hypertension.
本研究旨在通过组织形态计量学、免疫组织化学和超微结构研究α-硫辛酸对残余肾模型腹主动脉及高血压的影响。
我们采用残余肾模型手术将大鼠肾组织量减少至5/6。大鼠分为4组：第1组为对照组，第2组为硫辛酸组，第3组为5/6肾切除组，第4组为5/6肾切除+硫辛酸治疗组。第2组和第4组大鼠通过口服灌胃给予100 mg/kg硫辛酸溶液，持续8周。实验结束时，监测系统平均血压。然后，取出主动脉组织并固定。经过常规组织学处理后，对组织切片进行组织化学、免疫组织化学（I型血管紧张素受体、血管内皮生长因子、α-平滑肌肌动蛋白）和超微结构检查。
5/6肾切除组的血压测量值显著高于其他组。在5/6肾切除+硫辛酸组中，测得的血压值和中膜厚度显著低于5/6肾切除组。在5/6肾切除+硫辛酸组中，可见主动脉壁厚度减小、弹性纤维结构规则以及弹性层更有序。与5/6肾切除组相比，5/6肾切除+硫辛酸组中I型血管紧张素受体、血管内皮生长因子、α-平滑肌肌动蛋白的表达降低。
在本研究中，我们发现α-硫辛酸可能是一种对继发性高血压靶器官效应有利的药物。
