Sinnott Francine A, Monte Leonardo G, Collares Thais F, De Matos Bruno M, Pacheco Diene B, Borsuk Sibele, Andreotti Renato, Hartleben Cláudia P
Laboratório de Imunodiagnóstico, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, CEP 96010-900, Pelotas, RS, P.O. Box 354, Brazil.
Curr Microbiol. 2015 Mar;70(3):429-32. doi: 10.1007/s00284-014-0737-y. Epub 2014 Nov 29.
Neospora caninum is the etiologic agent of neosporosis, which leads to economic impacts on cattle industry. The reference method for serodiagnosis of neosporosis is the indirect fluorescent antibody test (IFAT). However, IFAT is laborious, expensive, and is not practicable in high throughput screening. In order to facilitate the serological diagnosis of neosporosis, we developed a blocking enzyme-linked immunosorbent assay (b-ELISA) based on NcSRS2 recombinant protein (rNcSRS2) and polyclonal antibodies against rNcSRS2 (b-ELISA/rNcSRS2). Compared to IFAT, b-ELISA/rNcSRS2 showed 93.7 % accuracy (98.7 % sensitivity and 88.7 % specificity), suggesting its potential as diagnostic assay to detect N. caninum antibodies in cattle sera.
犬新孢子虫是新孢子虫病的病原体，会给养牛业带来经济影响。新孢子虫病血清学诊断的参考方法是间接荧光抗体试验（IFAT）。然而，IFAT操作繁琐、成本高昂，且不适用于高通量筛查。为了便于新孢子虫病的血清学诊断，我们基于NcSRS2重组蛋白（rNcSRS2）和抗rNcSRS2多克隆抗体开发了一种阻断酶联免疫吸附测定法（b-ELISA）（b-ELISA/rNcSRS2）。与IFAT相比，b-ELISA/rNcSRS2的准确率为93.7%（敏感性为98.7%，特异性为88.7%），表明其作为检测牛血清中犬新孢子虫抗体诊断方法的潜力。
