de Castro Gabriela Salim, Andreoli María Florencia, Illesca Paola G, Payão Ovídio Paula, Bernal Claudio A, Jordão Alceu A, Vannucchi Helio
Department of Internal Medicine, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP. Brazil..
Food Science and Nutrition, Department of Biological Sciences, School of Biochemistry and Biological Sciences, Universidad Nacional del Litoral, Santa Fe. Argentina..
Nutr Hosp. 2014 Dec 1;30(6):1303-12. doi: 10.3305/nh.2014.30.6.7945.
There is no consensus about the effects of conjugated linoleic acid (CLA) on lipid metabolism, especially in animals fed a high-fat diet. Therefore, the objective of the present study was to evaluate the incorporation of CLA isomers into serum, liver and adipose tissue, as well as the oxidative stress generated in rats refed with high-fat diets after a 48 hour fast. Rats were refed with diets containing soybean oil, rich in linoleic acid [7% (Control Group - C) or 20% (LA Group)], CLA [CLA Group - 20% CLA mixture (39.32 mole% c9,t11-CLA and 40.59 mole% t10,c12- CLA)], soybean oil + CLA (LA+CLA Group - 15.4% soybean oil and 4.6% CLA) or animal fat (AF, 20% lard). The CLA group showed lower weight gain and liver weight after refeeding, as well as increased serum cholesterol. The high dietary fat intake induced fat accumulation and an increase in -tocopherol in the liver, which were not observed in the CLA group. Circulating -tocopherol was increased in the CLA and CLA+LA groups. The high- fat diets reduced liver catalase activity. CLA isomers were incorporated into serum and tissues. In this shortterm refeeding experimental model, CLA prevented hepatic fat accumulation, although it produced an increase in serum cholesterol.
关于共轭亚油酸（CLA）对脂质代谢的影响尚无定论，尤其是在高脂饮食喂养的动物中。因此，本研究的目的是评估CLA异构体在血清、肝脏和脂肪组织中的掺入情况，以及禁食48小时后再喂高脂饮食的大鼠体内产生的氧化应激。给大鼠重新喂食含有大豆油（富含亚油酸，[7%（对照组 - C）或20%（LA组）]）、CLA[CLA组 - 20% CLA混合物（39.32摩尔% c9,t11 - CLA和40.59摩尔% t10,c12 - CLA）]、大豆油 + CLA（LA + CLA组 - 15.4%大豆油和4.6% CLA）或动物脂肪（AF，20%猪油）的饮食。CLA组在重新喂食后体重增加和肝脏重量较低，血清胆固醇升高。高膳食脂肪摄入导致脂肪积累和肝脏中α-生育酚增加，而CLA组未观察到这种情况。CLA组和CLA + LA组循环中的α-生育酚增加。高脂饮食降低了肝脏过氧化氢酶活性。CLA异构体掺入血清和组织中。在这个短期再喂养实验模型中，CLA可预防肝脏脂肪积累，尽管它会使血清胆固醇升高。
