Bezan Priscila Nogueira, Holland Héric, de Castro Gabriela Salim, Cardoso João Felipe Rito, Ovidio Paula Payão, Calder Philip C, Jordao Alceu Afonso
Department of Internal Medicine, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Av. Bandeirantes 3900, Ribeirão Preto SP 14 049-900, Brazil.
Human Development and Health Academic Unit, Faculty of Medicine, University of Southampton, Southampton SO16 6YD, UK.
Exp Clin Endocrinol Diabetes. 2018 Jun;126(6):379-386. doi: 10.1055/s-0043-118348. Epub 2018 Jan 31.
Obesity and related diseases are becoming more prevalent. Conjugated linoleic acid (CLA) might be a useful coadjutant treatment helping to decrease fat mass. However, the precise impact of CLA is unclear because the decreased body fat mass is followed by an increase in insulin resistance. This study aimed to evaluate some of the consequences of a high dose of CLA in rats fed a normal low fat or a high fat diet for 30 days. Male Wistar rats were separated into 4 groups (each n = 10): Control group receiving 7% fat (soybean oil); CLA group receiving 4% soybean oil and 3% CLA mixture; animal fat (AF) group, receiving 45% fat (lard); and animal fat plus CLA (AF+CLA) group, receiving 42% lard and 3% CLA mixture. The CLA mixture contained 39.32 mole% c9,t11-CLA and 40.50 mole% t10,c12-CLA. After 30 days, both CLA groups (CLA and AF+CLA groups) developed insulin resistance, with an increase in glucose in the fasting state and in an insulin tolerance test. The CLA group had increased liver weight and percentage of saturated fatty acids in liver and adipose tissue. Feeding the high fat diet resulted in increased hepatic triacylglycerol accumulation and this was exacerbated by dietary CLA. It is concluded that a high dose of CLA mixture increases insulin resistance and exacerbates hepatic steatosis when combined with a high fat diet.
肥胖及相关疾病正变得越来越普遍。共轭亚油酸（CLA）可能是一种有助于减少脂肪量的有用辅助治疗手段。然而，CLA的确切影响尚不清楚，因为体脂量减少后会伴随胰岛素抵抗增加。本研究旨在评估高剂量CLA对喂食正常低脂或高脂饮食30天的大鼠的一些影响。雄性Wistar大鼠被分为4组（每组n = 10）：对照组给予7%脂肪（大豆油）；CLA组给予4%大豆油和3% CLA混合物；动物脂肪（AF）组，给予45%脂肪（猪油）；动物脂肪加CLA（AF + CLA）组，给予42%猪油和3% CLA混合物。CLA混合物含有39.32摩尔%的c9,t11 - CLA和40.50摩尔%的t10,c12 - CLA。30天后，两个CLA组（CLA组和AF + CLA组）均出现胰岛素抵抗，空腹状态下血糖升高，且在胰岛素耐量试验中也升高。CLA组肝脏重量增加，肝脏和脂肪组织中饱和脂肪酸百分比增加。喂食高脂饮食导致肝脏三酰甘油积累增加，而饮食中的CLA会加剧这种情况。得出的结论是，高剂量CLA混合物与高脂饮食联合使用时会增加胰岛素抵抗并加剧肝脏脂肪变性。
