Murcha Monika W, Narsai Reena, Devenish James, Kubiszewski-Jakubiak Szymon, Whelan James
Australian Research Council Centre of Excellence in Plant Energy Biology, Bayliss Building M316, University of Western Australia, 35 Stirling Highway, Crawley 6009, Western Australia, Australia
Department of Botany, Australian Research Council Centre of Excellence in Plant Energy Biology, School of Life Science, La Trobe University, Bundoora 3083, Victoria, Australia.
Plant Cell Physiol. 2015 Jan;56(1):e10. doi: 10.1093/pcp/pcu186. Epub 2014 Nov 29.
In the 2 billion years since the endosymbiotic event that gave rise to mitochondria, variations in mitochondrial protein import have evolved across different species. With the genomes of an increasing number of plant species sequenced, it is possible to gain novel insights into mitochondrial protein import pathways. We have generated the Mitochondrial Protein Import Components (MPIC) Database (DB; http://www.plantenergy.uwa.edu.au/applications/mpic) providing searchable information on the protein import apparatus of plant and non-plant mitochondria. An in silico analysis was carried out, comparing the mitochondrial protein import apparatus from 24 species representing various lineages from Saccharomyces cerevisiae (yeast) and algae to Homo sapiens (human) and higher plants, including Arabidopsis thaliana (Arabidopsis), Oryza sativa (rice) and other more recently sequenced plant species. Each of these species was extensively searched and manually assembled for analysis in the MPIC DB. The database presents an interactive diagram in a user-friendly manner, allowing users to select their import component of interest. The MPIC DB presents an extensive resource facilitating detailed investigation of the mitochondrial protein import machinery and allowing patterns of conservation and divergence to be recognized that would otherwise have been missed. To demonstrate the usefulness of the MPIC DB, we present a comparative analysis of the mitochondrial protein import machinery in plants and non-plant species, revealing plant-specific features that have evolved.
自产生线粒体的内共生事件发生后的20亿年里，线粒体蛋白输入的变异在不同物种间不断进化。随着越来越多植物物种的基因组被测序，人们有可能对线粒体蛋白输入途径获得新的认识。我们创建了线粒体蛋白输入组件（MPIC）数据库（网址：http://www.plantenergy.uwa.edu.au/applications/mpic），提供有关植物和非植物线粒体蛋白输入装置的可搜索信息。我们进行了一项计算机分析，比较了来自24个物种的线粒体蛋白输入装置，这些物种代表了从酿酒酵母（酵母）、藻类到智人（人类）以及高等植物（包括拟南芥、水稻和其他最近测序的植物物种）的各种谱系。对这些物种中的每一个都进行了广泛搜索并手动组装，以便在MPIC数据库中进行分析。该数据库以用户友好的方式呈现了一个交互式图表，允许用户选择他们感兴趣的输入组件。MPIC数据库提供了丰富的资源，有助于对线粒体蛋白输入机制进行详细研究，并能识别出那些否则可能会被遗漏的保守和差异模式。为了证明MPIC数据库的实用性，我们对植物和非植物物种的线粒体蛋白输入机制进行了比较分析，揭示了已进化出的植物特异性特征。
