秋水仙和长春花提取物对IFN-γ诱导的HaCaT人角质形成细胞中角蛋白17表达的影响。

Effect of Gloriosa superba and Catharanthus roseus Extracts on IFN-γ-Induced Keratin 17 Expression in HaCaT Human Keratinocytes.

作者信息

Pattarachotanant Nattaporn, Rakkhitawatthana Varaporn, Tencomnao Tewin

机构信息

Ph.D. Program in Clinical Biochemistry and Molecular Medicine, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand.

Center for Excellence in Omics-Nano Medical Technology Department Project, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand.

出版信息

Evid Based Complement Alternat Med. 2014;2014:249367. doi: 10.1155/2014/249367. Epub 2014 Nov 10.

DOI:10.1155/2014/249367

PMID:25435888

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4243713/

Abstract

Gloriosa superba and Catharanthus roseus are useful in traditional medicine for treatment of various skin diseases and cancer. However, their molecular effect on psoriasis has not been investigated. In this study, the effect of ethanol extracts derived from G. superba leaves and C. roseus stems on the expression of psoriatic marker, keratin 17 (K17), was investigated in human keratinocytes using biochemical and molecular experimental approaches. Both extracts could reduce the expression of K17 in a dose-dependent manner through JAK/STAT pathway as demonstrated by an observation of reduced phosphorylation of STAT3 (p-STAT3). The inhibitory activity of G. superba extract was more potent than that of C. roseus. The Pearson's correlation between K17 and cell viability was shown positive. Taken together, the extracts of G. superba and C. roseus may be developed as alternative therapies for psoriasis.

摘要

大花百部和长春花在传统医学中可用于治疗各种皮肤病和癌症。然而，它们对银屑病的分子作用尚未得到研究。在本研究中，采用生化和分子实验方法，研究了大花百部叶和长春花茎的乙醇提取物对人角质形成细胞中银屑病标志物角蛋白17（K17）表达的影响。两种提取物均可通过JAK/STAT途径以剂量依赖的方式降低K17的表达，这一点通过观察STAT3磷酸化（p-STAT3）的降低得到了证实。大花百部提取物的抑制活性比长春花提取物更强。K17与细胞活力之间的皮尔逊相关性呈阳性。综上所述，大花百部和长春花的提取物可能被开发为银屑病的替代疗法。