Bonnekoh B, Huerkamp C, Wevers A, Geisel J, Sebök B, Bange F C, Greenhalgh D A, Böttger E C, Krieg T, Mahrle G
Department of Dermatology, University of Cologne, Germany.
J Invest Dermatol. 1995 Jan;104(1):58-61. doi: 10.1111/1523-1747.ep12613492.
The immortalized human keratinocyte cell line HaCaT was used to assess the effect of interferon-gamma (IFN-gamma) on expression of keratin K17. Both IFN-gamma and K17 have been implicated in the pathophysiology of psoriasis. Western and quantitative enzyme-linked immunosorbent assay analyses demonstrated increasing induction of K17 protein by 48 h exposure to IFN-gamma at concentrations of 10, 50, and 250 U/ml. At 50 U/ml IFN-gamma, immunohistochemical analysis revealed numerous K17-positive foci, whereas in situ hybridization demonstrated K17 message in the majority of cells. In addition, at low (5 U/ml) concentrations of IFN-gamma, cell proliferation and protein synthesis decreased, as determined by 3H-thymidine labeling and 14C-amino acid uptake. These data suggest that aberrant K17 expression observed in psoriatic lesions may be a consequence of IFN-gamma overexpression, and that the HaCaT cell line may be a useful in vitro model system to elucidate the underlying mechanisms.
永生化人角质形成细胞系HaCaT用于评估干扰素-γ（IFN-γ）对角蛋白K17表达的影响。IFN-γ和K17均与银屑病的病理生理学有关。蛋白质免疫印迹和定量酶联免疫吸附测定分析表明，在10、50和250 U/ml浓度下暴露于IFN-γ 48小时，K17蛋白的诱导增加。在50 U/ml IFN-γ时，免疫组织化学分析显示大量K17阳性灶，而原位杂交显示大多数细胞中有K17信息。此外，在低浓度（5 U/ml）的IFN-γ下，通过3H-胸腺嘧啶核苷标记和14C-氨基酸摄取测定，细胞增殖和蛋白质合成减少。这些数据表明，银屑病皮损中观察到的K17异常表达可能是IFN-γ过表达的结果，并且HaCaT细胞系可能是阐明潜在机制的有用体外模型系统。
