磷脂酰乙醇胺转化为磷脂酰胆碱过程中两种甲基转移酶的鉴定及特性
Identification and properties of two methyltransferases in conversion of phosphatidylethanolamine to phosphatidylcholine.
作者信息
Hirata F, Viveros O H, Diliberto E J, Axelrod J
出版信息
Proc Natl Acad Sci U S A. 1978 Apr;75(4):1718-21. doi: 10.1073/pnas.75.4.1718.
Abstract
Two methyltransferases involved in the methylation of phosphatidylethanolamine to form phosphatidylcholine were demonstrated in a microsomal fraction of bovine adrenal medulla. The first methyltransferase catalyzes the methylation of phosphatidylethanolamine to form phosphatidyl-N-monomethylethanolamine. This enzyme has an optimum pH of 6.5, a low Km for S-adenosyl-L-methionine (1.4 micron), and an absolute requirement for Mg2+. The second methyltransferase catalyzes the two successive methylations of phodphatidyl-N-monomethylethanolamine to phosphatidyl-N,N-dimethylethanolamine and phosphatidylcholine. In contrast to the first methyltransferase, it has an optimum pH of 10 and a high Km for S-adenosyl-L-methionine (0.1 mM) and does not require Mg2+.
摘要
在牛肾上腺髓质的微粒体部分发现了两种参与磷脂酰乙醇胺甲基化形成磷脂酰胆碱的甲基转移酶。第一种甲基转移酶催化磷脂酰乙醇胺甲基化形成磷脂酰 - N - 单甲基乙醇胺。这种酶的最适pH为6.5，对S - 腺苷 - L - 甲硫氨酸的Km值较低（1.4微米），并且绝对需要Mg2 +。第二种甲基转移酶催化磷脂酰 - N - 单甲基乙醇胺连续两次甲基化形成磷脂酰 - N，N - 二甲基乙醇胺和磷脂酰胆碱。与第一种甲基转移酶相反，它的最适pH为10，对S - 腺苷 - L - 甲硫氨酸的Km值较高（0.1 mM），并且不需要Mg2 +。
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本文引用的文献
1
STUDIES ON THE METHYLATION OF ETHANOLAMINE PHOSPHATIDES BY LIVER PREPARATIONS.肝脏制剂对乙醇胺磷脂甲基化作用的研究。
Arch Biochem Biophys. 1965 Jan;109:110-5. doi: 10.1016/0003-9861(65)90294-8.
2
The enzymatic conversion of phospholipid ethanolamine to phospholipid choline in rat liver.大鼠肝脏中磷脂酰乙醇胺向磷脂酰胆碱的酶促转化。
J Biol Chem. 1961 Mar;236:673-9.
3
The function of cytidine coenzymes in the biosynthesis of phospholipides.胞苷辅酶在磷脂生物合成中的作用。
J Biol Chem. 1956 Sep;222(1):193-214.
4
5
Methylation of ethanolamine phosphatides by microsomes from normal and mutant strains of Neurospora crassa.粗糙脉孢菌正常菌株和突变菌株微粒体对乙醇胺磷脂的甲基化作用。
J Biol Chem. 1967 Jan 25;242(2):238-42.
6
Pituitary gland: enzymic formation of methanol from S-adenosylmethionine.垂体：由S-腺苷甲硫氨酸酶促形成甲醇。
Science. 1965 Nov 12;150(3698):892-3. doi: 10.1126/science.150.3698.892.
7
Subcellualr distribution of protein carboxymethylase and its endogenous substrates in the adrenal medulla: possible role in excitation-secretion coupling.肾上腺髓质中蛋白质羧甲基酶及其内源性底物的亚细胞分布：在兴奋-分泌偶联中的可能作用。
Proc Natl Acad Sci U S A. 1976 Nov;73(11):4050-4. doi: 10.1073/pnas.73.11.4050.
8
Topological asymmetry of phospholipids in membranes.膜中磷脂的拓扑不对称性。
Science. 1977 Jul 15;197(4300):224-30. doi: 10.1126/science.327544.
9
Regional and subcellular distribution of protein carboxymethylase in brain and other tissues.蛋白质羧甲基酶在脑及其他组织中的区域和亚细胞分布。
J Neurochem. 1976 Jun;26(6):1159-65. doi: 10.1111/j.1471-4159.1976.tb07001.x.
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