通过口服仙人掌黏液逆转大鼠乙醇诱导的慢性胃炎中的胃黏膜改变。
Reversing gastric mucosal alterations during ethanol-induced chronic gastritis in rats by oral administration of Opuntia ficus-indica mucilage.
作者信息
Vázquez-Ramírez Ricardo, Olguín-Martínez Marisela, Kubli-Garfias Carlos, Hernández-Muñoz Rolando
机构信息
Departamento de Biologia Celular y Fisiologia Instituto de Investigaciones Biomédicas. Universidad Nacional Autónoma de Mexico. Apdo. Postal 70-243. Mexico D.F. 04510, Mexico.
出版信息
World J Gastroenterol. 2006 Jul 21;12(27):4318-24. doi: 10.3748/wjg.v12.i27.4318.
AIM
To study the effect of mucilage obtained from cladodes of Opuntia ficus-indica (Cactaceae) on the healing of ethanol-induced gastritis in rats.
METHODS
Chronic gastric mucosa injury was treated with mucilage (5 mg/kg per day) after it was induced by ethanol. Lipid composition, activity of 5'-nucleotidase (a membrane-associated ectoenzyme) and cytosolic activities of lactate and alcohol dehydrogenases in the plasma membrane of gastric mucosa were determined. Histological studies of gastric samples from the experimental groups were included.
RESULTS
Ethanol elicited the histological profile of gastritis characterized by loss of the surface epithelium and infiltration of polymorphonuclear leukocytes. Phosphatidylcholine (PC) decreased and cholesterol content increased in plasma membranes of the gastric mucosa. In addition, cytosolic activity increased while the activity of alcohol dehydrogenases decreased. The administration of mucilage promptly corrected these enzymatic changes. In fact, mucilage readily accelerated restoration of the ethanol-induced histological alterations and the disturbances in plasma membranes of gastric mucosa, showing a univocal anti-inflammatory effect. The activity of 5'-nucleotidase correlated with the changes in lipid composition and the fluidity of gastric mucosal plasma membranes.
CONCLUSION
The beneficial action of mucilage seems correlated with stabilization of plasma membranes of damaged gastric mucosa. Molecular interactions between mucilage monosaccharides and membrane phospholipids, mainly PC and phosphatidylethanolamine (PE), may be the relevant features responsible for changing activities of membrane-attached proteins during the healing process after chronic gastric mucosal damage.
目的
研究从仙人掌科植物仙人掌肉质茎中提取的黏液对乙醇诱导的大鼠胃炎愈合的影响。
方法
乙醇诱导慢性胃黏膜损伤后，用黏液（每天5毫克/千克）进行治疗。测定胃黏膜质膜中的脂质组成、5'-核苷酸酶（一种膜相关外切酶）的活性以及乳酸脱氢酶和乙醇脱氢酶的胞质活性。纳入对实验组胃样本的组织学研究。
结果
乙醇引发了胃炎的组织学特征，表现为表面上皮细胞缺失和多形核白细胞浸润。胃黏膜质膜中的磷脂酰胆碱（PC）减少，胆固醇含量增加。此外，胞质活性增加，而乙醇脱氢酶的活性降低。黏液的给药迅速纠正了这些酶变化。事实上，黏液能迅速加速乙醇诱导的组织学改变和胃黏膜质膜紊乱的恢复，显示出明确的抗炎作用。5'-核苷酸酶的活性与脂质组成的变化以及胃黏膜质膜的流动性相关。
结论
黏液的有益作用似乎与受损胃黏膜质膜的稳定有关。黏液单糖与膜磷脂（主要是PC和磷脂酰乙醇胺（PE））之间的分子相互作用可能是慢性胃黏膜损伤后愈合过程中导致膜附着蛋白活性改变的相关特征。
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