McVoy M A, Adler S P
Department of Pediatrics, Children's Medical Center, Medical College of Virginia, Richmond.
J Infect Dis. 1989 Jul;160(1):1-10. doi: 10.1093/infdis/160.1.1.
Immunoblot analysis using mouse monoclonal antibody to human IgM detected IgM to cytomegalovirus (CMV)-specific proteins (150, 42, 38, 32, and 28 kDa) in 74 (38%) of 197 seropositive serum samples from 197 individuals in three subject groups: 43 surgical patients, 31 patients with solid tumors, and 123 healthy individuals. Logistic regression analysis revealed that the presence of IgM to CMV proteins was not associated with a specific subject group but was associated with age (P less than .0001), gender (P less than .005), and IgG titer (P less than .03). The rate of IgM positivity increased from 15% (8 of 54) for those less than 20 y to 63% (37 of 59) for those greater than 60 y. Twenty-three (29%) of 80 males were IgM-positive compared with 49 (44%) of 111 females. Immunoglobulin G titers to CMV were significantly associated with age, gender, and IgM positivity. These data suggest that CMV reactivation in immunocompetent seropositive individuals may occur frequently and increase with age.
使用抗人IgM小鼠单克隆抗体进行的免疫印迹分析，在来自三个受试者组（43名外科手术患者、31名实体瘤患者和123名健康个体）的197名个体的197份血清学阳性血清样本中，有74份（38%）检测到针对巨细胞病毒（CMV）特异性蛋白（150、42、38、32和28 kDa）的IgM。逻辑回归分析显示，针对CMV蛋白的IgM的存在与特定受试者组无关，但与年龄（P小于0.0001）、性别（P小于0.005）和IgG滴度（P小于0.03）有关。IgM阳性率从20岁以下人群的15%（54人中的8人）增加到60岁以上人群的63%（59人中的37人）。80名男性中有23名（29%）IgM呈阳性，而111名女性中有49名（44%）。针对CMV的免疫球蛋白G滴度与年龄、性别和IgM阳性显著相关。这些数据表明，免疫功能正常的血清学阳性个体中CMV再激活可能频繁发生且随年龄增加。
