重组痘苗病毒增强爱泼斯坦-巴尔病毒潜伏膜蛋白的表达。
Enhanced expression of the Epstein-Barr virus latent membrane protein by a recombinant vaccinia virus.
作者信息
Stewart J P, Hampson I N, Heinrich H W, Mackett M, Arrand J R
机构信息
Paterson Institute for Cancer Research, Manchester, U.K.
出版信息
J Gen Virol. 1989 May;70 ( Pt 5):1231-7. doi: 10.1099/0022-1317-70-5-1231.
The complete coding sequence of the Epstein-Barr virus strain B95-8 latent membrane protein (LMP) was cloned using a Raji cell cDNA library and genomic B95-8 DNA. The clone was characterized by sequencing and then used to make a recombinant vaccinia virus. This virus (VLMP) was shown to express a relatively high level of LMP in an authentic fashion. Antisera raised in rabbits against VLMP were shown to react with B95-8 LMP as well as cross-reacting with a 50K cellular protein.
利用拉吉细胞cDNA文库和B95 - 8基因组DNA克隆了爱泼斯坦-巴尔病毒B95 - 8株潜伏膜蛋白（LMP）的完整编码序列。通过测序对该克隆进行了表征，然后用于制备重组痘苗病毒。这种病毒（VLMP）被证明能以真实的方式表达相对高水平的LMP。用针对VLMP的兔抗血清显示，其能与B95 - 8 LMP反应，并且与一种50K细胞蛋白发生交叉反应。
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