Chase C C, Carter-Allen K, Lohff C, Letchworth G J
Department of Veterinary Science, University of Wisconsin-Madison 53706.
J Virol. 1990 Oct;64(10):4866-72. doi: 10.1128/JVI.64.10.4866-4872.1990.
We expressed the bovine herpesvirus 1 (BHV-1) glycoprotein IV (gIV) in bovine cells. The protein expressed was identical in molecular mass and antigenic reactivity to the native gIV protein but was localized in the cytoplasm. Expressing cells were partially resistant to BHV-1, herpes simplex virus, and pseudorabies virus, as shown by a 10- to 1,000-fold-lower number of plaques forming on these cells than on control cells. The level of resistance depended on the level of gIV expression and the type and amount of challenge virus. These data are consistent with previous reports by others that cellular expression of the BHV-1 gIV homologs, herpes simplex virus glycoprotein D, and pseudorabies virus glycoprotein gp50 provide partial resistance against infection with these viruses. We have extended these findings by showing that once BHV-1 enters gIV-expressing cells, it replicates and spreads normally, as shown by the normal size of BHV-1 plaques and the delayed but vigorous synthesis of viral proteins. Our data are consistent with the binding of BHV-1 gIV to a cellular receptor required for initial penetration by all three herpesviruses and interference with the function of that receptor molecule.
我们在牛细胞中表达了牛疱疹病毒1型（BHV-1）糖蛋白IV（gIV）。所表达的蛋白在分子量和抗原反应性方面与天然gIV蛋白相同，但定位于细胞质中。如在这些细胞上形成的噬斑数量比对照细胞低10至1000倍所示，表达细胞对BHV-1、单纯疱疹病毒和伪狂犬病病毒具有部分抗性。抗性水平取决于gIV的表达水平以及攻击病毒的类型和数量。这些数据与其他人之前的报道一致，即BHV-1 gIV同源物、单纯疱疹病毒糖蛋白D和伪狂犬病病毒糖蛋白gp50的细胞表达对这些病毒的感染提供部分抗性。我们通过表明一旦BHV-1进入表达gIV的细胞，它就会正常复制和传播，扩展了这些发现，如BHV-1噬斑的正常大小以及病毒蛋白延迟但旺盛的合成所示。我们的数据与BHV-1 gIV与所有三种疱疹病毒初始穿透所需的细胞受体结合并干扰该受体分子的功能一致。
