Liu Yu-Jia, Zhao Yu-Jun, Zhang Meng, Su Ping, Wang Xiu-Juan, Zhang Xia-Nan, Gao Wei, Huang Lu-Qi
School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China.
National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Molecules. 2014 Nov 27;19(12):19696-707. doi: 10.3390/molecules191219696.
Tripterygium wilfordii is a traditional Chinese medical plant used to treat rheumatoid arthritis and cancer. The main bioactive compounds of the plant are diterpenoids and triterpenoids. 3-Hydroxy-3-methylglutaryl-CoA synthase (HMGS) catalyses the reaction of acetoacetyl-CoA to 3-hydroxy-3-methylglutaryl-CoA, which is the first committed enzyme in the mevalonate (MVA) pathway. The sequence information of HMGS in Tripterygium wilfordii is a basic resource necessary for studying the terpenoids in the plant. In this paper, full-length cDNA encoding HMGS was isolated from Tripterygium wilfordii (abbreviated TwHMGS, GenBank accession number: KM978213). The full length of TwHMGS is 1814 bp, and the gene encodes a protein with 465 amino acids. Sequence comparison revealed that TwHMGS exhibits high similarity to HMGSs of other plants. The tissue expression patterns revealed that the expression level of TwHMGS is highest in the stems and lowest in the roots. Induced expression of TwHMGS can be induced by MeJA, and the expression level is highest 4 h after induction. The functional complement assays in the YML126C knockout yeast demonstrated that TwHMGS participates in yeast terpenoid biosynthesis.
雷公藤是一种用于治疗类风湿性关节炎和癌症的传统中药植物。该植物的主要生物活性化合物是二萜类和三萜类。3-羟基-3-甲基戊二酰辅酶A合酶（HMGS）催化乙酰乙酰辅酶A转化为3-羟基-3-甲基戊二酰辅酶A的反应，这是甲羟戊酸（MVA）途径中的第一个关键酶。雷公藤中HMGS的序列信息是研究该植物萜类化合物的必要基础资源。本文从雷公藤中分离出编码HMGS的全长cDNA（缩写为TwHMGS，GenBank登录号：KM978213）。TwHMGS全长1814 bp，该基因编码一个含有465个氨基酸的蛋白质。序列比较显示，TwHMGS与其他植物的HMGS具有高度相似性。组织表达模式显示，TwHMGS在茎中的表达水平最高，在根中的表达水平最低。TwHMGS的诱导表达可被茉莉酸甲酯（MeJA）诱导，诱导后4小时表达水平最高。在YML126C基因敲除酵母中的功能互补试验表明，TwHMGS参与酵母萜类生物合成。
