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雷公藤法尼基焦磷酸合酶的分子克隆与鉴定

Molecular Cloning and Characterisation of Farnesyl Pyrophosphate Synthase from Tripterygium wilfordii.

作者信息

Zhao Yu-Jun, Chen Xin, Zhang Meng, Su Ping, Liu Yu-Jia, Tong Yu-Ru, Wang Xiu-Juan, Huang Lu-Qi, Gao Wei

机构信息

Capital Medical University School of Traditional Chinese Medicine, Capital Medical University, Beijing, People's Republic of China.

National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, People's Republic of China.

出版信息

PLoS One. 2015 May 4;10(5):e0125415. doi: 10.1371/journal.pone.0125415. eCollection 2015.

DOI:10.1371/journal.pone.0125415
PMID:25938487
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4418688/
Abstract

Farnesylpyrophosphate synthase (FPS) catalyzes the biosynthesis of farnesyl pyrophosphate (FPP), which is an important precursor of sesquiterpenoids such as artemisinin and wilfordine. In the present study, we report the molecular cloning and characterization of two full-length cDNAs encoding FPSs from Tripterygium wilfordii (TwFPSs). TwFPSs maintained their capability to synthesise FPP in vitro when purified as recombinant proteins from E. coli. Consistent with the endogenous role of FPS in FPP biosynthesis, TwFPSs were highly expressed in T. wilfordii roots, and were up-regulated upon methyl jasmonate (MeJA) treatment. The global gene expression profiles suggested that the TwFPSs might play an important regulatory role interpenoid biosynthesis in T. wilfordii, laying the groundwork for the future study of the synthetic biology of natural terpene products.

摘要

法尼基焦磷酸合酶（FPS）催化法尼基焦磷酸（FPP）的生物合成，FPP是青蒿素和雷公藤碱等倍半萜类化合物的重要前体。在本研究中，我们报道了从雷公藤中克隆和鉴定的两个编码FPS的全长cDNA（TwFPSs）。当从大肠杆菌中纯化重组蛋白时，TwFPSs在体外保持了合成FPP的能力。与FPS在FPP生物合成中的内源性作用一致，TwFPSs在雷公藤根中高表达，并在茉莉酸甲酯（MeJA）处理后上调。全局基因表达谱表明，TwFPSs可能在雷公藤的萜类生物合成中发挥重要的调节作用，为天然萜类产物合成生物学的未来研究奠定了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35f2/4418688/62189a56c44a/pone.0125415.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35f2/4418688/6278e2b94697/pone.0125415.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35f2/4418688/1f6fc0552914/pone.0125415.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35f2/4418688/465b861c1453/pone.0125415.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35f2/4418688/323631b43a96/pone.0125415.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35f2/4418688/32ed29e8864b/pone.0125415.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35f2/4418688/001f6dc18367/pone.0125415.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35f2/4418688/62189a56c44a/pone.0125415.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35f2/4418688/6278e2b94697/pone.0125415.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35f2/4418688/1f6fc0552914/pone.0125415.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35f2/4418688/465b861c1453/pone.0125415.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35f2/4418688/323631b43a96/pone.0125415.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35f2/4418688/32ed29e8864b/pone.0125415.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35f2/4418688/001f6dc18367/pone.0125415.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35f2/4418688/62189a56c44a/pone.0125415.g007.jpg

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