水痘带状疱疹病毒临床分离株的限制性内切酶分析比较

Comparative restriction endonuclease analysis of varicella-zoster virus clinical isolates.

作者信息

Takayama M, Takayama N, Kameoka Y, Hachimori K, Kaneda K, Minamitani M

机构信息

National Institute of Health, Japan, Tokyo.

出版信息

Med Microbiol Immunol. 1989;178(2):61-7. doi: 10.1007/BF00203301.

DOI:10.1007/BF00203301

PMID:2543893

Abstract

The DNAs of 67 isolates of varicella-zoster virus (VZV) obtained from 31 individuals were compared by restriction endonuclease analysis using BamHI, EcoRI, PstI and SmaI. All of the epidemiologically unrelated 26 isolates could be differentiated using SmaI and another one or two enzymes. However, the DNA cleavage profiles of multiple VZV isolates from the same patient and the isolates from a group of patients who were infected with VZV from the same source were found to be identical to each other, as reported previously. No patients were found who were simultaneously infected with different VZV strains. Moreover, VZV showed no change in DNA fragment profiles after serial passages not only through human embryonic lung cells but also through patients.

摘要

使用BamHI、EcoRI、PstI和SmaI通过限制性内切酶分析比较了从31名个体获得的67株水痘带状疱疹病毒（VZV）的DNA。所有26株流行病学上不相关的分离株都可以使用SmaI和另外一种或两种酶进行区分。然而，正如之前报道的那样，发现来自同一患者的多个VZV分离株以及来自同一来源感染VZV的一组患者的分离株的DNA切割图谱彼此相同。未发现同时感染不同VZV毒株的患者。此外，VZV不仅在通过人胚肺细胞连续传代后，而且在通过患者传代后，DNA片段图谱均未发生变化。