Hondo R, Yogo Y, Kurata T, Aoyama Y
Arch Virol. 1987;93(1-2):1-12. doi: 10.1007/BF01313890.
We have used 12 restriction enzymes to analyse the DNA of 24 clinical isolates of VZV derived from 12 patients in order to compare isolates derived from different individuals and derived serially from the same individual. As reported previously, only a small proportion of the isolates differed with respect to the presence or absence of restriction sites. However, we found that the size of DNA fragments generated from all the isolates derived from different patients varied in any of four regions, one of which was first recognized in this study. In one case, where multiple isolates recovered from the same individual were analysed, each was distinguished from the others not only by differences in the variable regions but also by the presence or absence of a restriction site in a nonvariable region. This suggests that multiple strains of VZV can be present in the same human host.
我们使用了12种限制性内切酶来分析从12名患者身上分离出的24株水痘带状疱疹病毒（VZV）临床分离株的DNA，以便比较来自不同个体以及同一患者连续分离出的分离株。如先前报道，只有一小部分分离株在限制性酶切位点的有无方面存在差异。然而，我们发现，来自不同患者的所有分离株产生的DNA片段大小在四个区域中的任何一个区域都有所不同，其中一个区域是本研究首次发现的。在一个案例中，对从同一患者身上分离出的多个分离株进行分析时，每个分离株不仅在可变区存在差异，而且在非可变区也存在或不存在限制性酶切位点，从而相互区分。这表明同一人类宿主中可能存在多种VZV毒株。
