Department of Microbial Engineering, Konkuk University, Seoul 143-701, South Korea.
Department of Chemical and Biochemical Engineering, Dongguk University-Seoul, Seoul 100-715, South Korea.
Carbohydr Polym. 2015 Jan 22;115:223-9. doi: 10.1016/j.carbpol.2014.08.096. Epub 2014 Sep 6.
Wood component-based composite hydrogels have potential applications in biomedical fields owing to their low cost, biodegradability, and biocompatibility. The controllable properties of wood mimetic composites containing three major wood components are useful for enzyme immobilization. Here, lipase from Candida rugosa was entrapped in wood mimetic beads containing cellulose, xylan, and lignin by dissolving wood components with lipase in [Emim][Ac], followed by reconstitution. Lipase entrapped in cellulose/xylan/lignin beads in a 5:3:2 ratio showed the highest activity; this ratio is very similar to that in natural wood. The lipase entrapped in various wood mimetic beads showed increased thermal and pH stability. The half-life times of lipase entrapped in cellulose/alkali lignin hydrogel were 31- and 82-times higher than those of free lipase during incubation under denaturing conditions of high temperature and low pH, respectively. Owing to their biocompatibility, biodegradability, and controllable properties, wood mimetic hydrogel beads can be used to immobilize various enzymes for applications in the biomedical, bioelectronic, and biocatalytic fields.
基于木材成分的复合水凝胶由于其低成本、可生物降解性和生物相容性，在生物医学领域具有潜在的应用。含有三种主要木材成分的仿生复合材料的可控性能可用于酶固定化。在这里，通过将含有纤维素、木聚糖和木质素的木材成分与脂肪酶溶解在[Emim][Ac]中，然后再进行重组，将来自假丝酵母的脂肪酶包埋在仿生珠中。在纤维素/木聚糖/木质素珠中以 5:3:2 比例包埋的脂肪酶显示出最高的活性；这个比例与天然木材非常相似。在各种木材仿生珠中包埋的脂肪酶显示出热稳定性和 pH 稳定性的提高。在高温和低 pH 变性条件下孵育时，包埋在纤维素/堿木质素水凝胶中的脂肪酶的半衰期比游离脂肪酶分别高 31 倍和 82 倍。由于其生物相容性、可生物降解性和可控性能，木材仿生水凝胶珠可用于固定各种酶，应用于生物医学、生物电子和生物催化领域。
