Ban Eunmi, Song Eun Joo
Molecular Recognition Research Center, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea.
Molecular Recognition Research Center, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea.
Anal Chim Acta. 2014 Dec 10;852:1-7. doi: 10.1016/j.aca.2014.08.034. Epub 2014 Aug 21.
MicroRNAs (miRNAs) are short noncoding RNAs that conduct important roles in many cellular processes such as development, proliferation, differentiation, and apoptosis. In particular, circulating miRNAs have been proposed as biomarkers for cancer, diabetes, cardiovascular disease, and other illnesses. Therefore, determination of miRNA expression levels in various biofluids is important for the investigation of biological processes in health and disease and for discovering their potential as new biomarkers and drug targets. Capillary electrophoresis (CE) is emerging as a useful analytical tool for analyzing miRNA because of its simple sample preparation steps and efficient resolution of a diverse size range of compounds. In particular, CE with laser-induced fluorescence detection is a promising and relatively rapidly developing tool with the potential to provide high sensitivity and specificity in the analysis of miRNAs. This paper covers a short overview of the recent developments and applications of CE systems in miRNA studies in biological and biomedical areas.
微小RNA（miRNA）是短链非编码RNA，在许多细胞过程如发育、增殖、分化和凋亡中发挥重要作用。特别是，循环miRNA已被提议作为癌症、糖尿病、心血管疾病和其他疾病的生物标志物。因此，测定各种生物流体中miRNA的表达水平对于研究健康和疾病中的生物学过程以及发现它们作为新生物标志物和药物靶点的潜力非常重要。毛细管电泳（CE）因其简单的样品制备步骤和对各种大小范围化合物的高效分离能力，正逐渐成为分析miRNA的有用分析工具。特别是，带有激光诱导荧光检测的CE是一种有前途且发展相对迅速的工具，有潜力在miRNA分析中提供高灵敏度和特异性。本文简要概述了CE系统在生物和生物医学领域miRNA研究中的最新进展和应用。
