Department of Joint Surgery, No 89 Hospital of People's Liberation Army, Weifang 261021, China.
Department of Joint Surgery, No 89 Hospital of People's Liberation Army, Weifang 261021, China.
Asian Pac J Trop Med. 2014 Nov;7(11):895-9. doi: 10.1016/S1995-7645(14)60156-5. Epub 2014 Oct 22.
To observe the effect of recombinant human bone morphogenetic protein 2/poly-lactide-co-glycolic acid (rhBMP-2/PLGA) with core decompression on repair of rabbit femoral head necrosis.
Bilateral femoral head necrosis models of rabbit were established by steroid injection. A total of 48 rabbits (96 femoral head necrosis) were randomly divided into 4 groups: Group A, control group with12 rabbits, 24 femoral head necrosis; Group B, treated with rhBMP-2/PLGA implantation after core depression, with 12 rabbits, 24 femoral head necrosis; Group C, treated with rhBMP-2 implantation after core depression, with 12 rabbits, 24 femoral head necrosis; Group D treated with core depression group without implantation, with 12 rabbits, 24 femoral head necrosis. All animals were sacrificed after 12 weeks. The ability of repairing bone defect was evaluated by X-ray radiograph. Bone mineral density analysis of the defect regions were used to evaluate the level of ossification. The morphologic change and bone formation was assessed by HE staining. The angiogenesis was evaluated by VEGF immunohistochemistry.
The osteogenetic ability and quality of femoral head necrosis in group B were better than those of other groups after 12 weeks by X-ray radiograph and morphologic investigation. And the angiogenesis in group B was better than other groups. Group C had similar osteogenetic quality of femoral head necrosis and angiogenesis with group D.
The treatment of rhBMP-2/PLGA implantation after core depression can promote the repair of rabbit femoral head necrosis. It is a promising and efficient synthetic bone material to treat the femoral head necrosis.
观察 rhBMP-2/PLGA 复合骨水泥加髓芯减压对兔股骨头坏死修复的影响。
采用激素注射法建立双侧兔股骨头坏死模型。将 48 只兔（96 个股骨头坏死）随机分为 4 组：A 组为对照组，12 只兔，24 个股骨头坏死；B 组为 rhBMP-2/PLGA 复合骨水泥加髓芯减压组，12 只兔，24 个股骨头坏死；C 组为 rhBMP-2 加髓芯减压组，12 只兔，24 个股骨头坏死；D 组为单纯髓芯减压组，12 只兔，24 个股骨头坏死。所有动物在 12 周后处死。X 线片评估骨缺损修复能力，缺损区骨密度分析评估成骨水平，HE 染色评估形态学变化和骨形成，VEGF 免疫组化评估血管生成。
12 周后 X 线片和形态学观察显示，B 组股骨头坏死的成骨能力和质量优于其他组；B 组的血管生成也优于其他组。C 组与 D 组的股骨头坏死成骨质量和血管生成相似。
rhBMP-2/PLGA 复合骨水泥加髓芯减压治疗能促进兔股骨头坏死的修复，是一种有前途的、有效的治疗股骨头坏死的合成骨材料。
