碱性预处理和酸提取条件对草鱼（Ctenopharyngodon idella）皮中酸溶性胶原蛋白的影响。
Effects of alkaline pretreatments and acid extraction conditions on the acid-soluble collagen from grass carp (Ctenopharyngodon idella) skin.
机构信息
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu Province 214122, China; Synergetic Innovation Center of Food Safety and Nutrition, Wuxi, Jiangsu Province 214122, China.
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu Province 214122, China.
出版信息
Food Chem. 2015 Apr 1;172:836-43. doi: 10.1016/j.foodchem.2014.09.147. Epub 2014 Oct 5.
This study investigated the effects of alkaline pretreatments and acid extraction conditions on the production of acid-soluble collagen (ASC) from grass carp skin. For alkaline pretreatment, 0.05 and 0.1M NaOH removed non-collagenous proteins without significant loss of ASC at 4, 10, 15 and 20 °C; while 0.2 and 0.5M NaOH caused significant loss of ASC, and 0.5M NaOH caused structural modification of ASC at 15 and 20 °C. For acid extraction at 4, 10, 15 and 20 °C, ASC was partly extracted by 0.1 and 0.2M acetic acid, while 0.5 and 1.0M acetic acid resulted in almost complete extraction. The processing conditions involving 0.05-0.1M NaOH for pretreatment, 0.5M acetic acid for extraction and 4-20 °C for both pretreatment and extraction, produced ASC with the structural integrity being well maintained and hence were recommended to prepare ASC from grass carp skin in practical application.
本研究探讨了碱性预处理和酸提取条件对草鱼皮酸溶性胶原蛋白（ASC）生产的影响。对于碱性预处理，0.05 和 0.1M NaOH 在 4、10、15 和 20°C 下去除非胶原蛋白而不显著损失 ASC；而 0.2 和 0.5M NaOH 则导致 ASC 显著损失，0.5M NaOH 在 15 和 20°C 下导致 ASC 的结构修饰。对于 4、10、15 和 20°C 的酸提取，0.1 和 0.2M 乙酸部分提取 ASC，而 0.5 和 1.0M 乙酸则几乎完全提取 ASC。预处理采用 0.05-0.1M NaOH，提取采用 0.5M 乙酸，预处理和提取温度为 4-20°C 的处理条件，制备的 ASC 结构完整性得到很好的保持，因此建议在实际应用中从草鱼皮制备 ASC。
Zotero 插件