Scheja Judith Maria, Sigurðarson Jens Jakob, Ólafsson Halldór Gunnar, Einarsson Hjörleifur
BioPol ehf, 545 Skagaströnd, Iceland.
Faculty of Natural Resource Sciences, University of Akureyri, 600 Akureyri, Iceland.
Mar Drugs. 2024 Nov 22;22(12):525. doi: 10.3390/md22120525.
Female lumpfish () are a primary target of commercial fishery for their roe, a substitute for caviar. The remaining carcasses are underutilized rest raw material. The pre-treatment and acid extraction conditions of collagen from lumpfish skins were optimized. Full factorial design was used to optimize the alkali pre-treatment conditions with NaOH. The optimal conditions were X = 0.1 M (NaOH concentration), X = 6 h (NaOH treatment time), X = 4 °C (treatment temperature) and X = 1:5 (/, solid to liquid ratio). Optimized conditions for collagen extraction with acetic acid were investigated using a Box-Behnken design. The result suggested a concentration of 0.9 M acetic acid, treatment temperature of 21 °C, a treatment time of 36 h in a total of 2 volumes of acid. Combined optimized effects resulted in a collagen yield of 45% (DW/DW) with high purity (>90%) and a high hydroxyproline content (7.9%). A scale-up experiment (starting with 45 kg) showed that the yield was somewhat lower (18-25%). Enzyme hydrolysis of skin after acetic acid extraction added another 23% (DW/DW) to the yield and enzyme hydrolysis of precleaned skins resulted in 60% (DW/DW).
雌性圆鳍鱼（）因其鱼卵（可替代鱼子酱）而成为商业渔业的主要捕捞对象。剩余的鱼体则是未得到充分利用的原材料。对圆鳍鱼皮胶原蛋白的预处理和酸提取条件进行了优化。采用全因子设计优化了用氢氧化钠进行碱预处理的条件。最佳条件为X = 0.1 M（氢氧化钠浓度）、X = 6小时（氢氧化钠处理时间）、X = 4℃（处理温度）以及X = 1:5（/，固液比）。使用Box-Behnken设计研究了用乙酸提取胶原蛋白的优化条件。结果表明，乙酸浓度为0.9 M、处理温度为21℃、处理时间为36小时，总共使用2倍体积的酸。综合优化效果使胶原蛋白产率达到45%（干重/干重），纯度高（>90%）且羟脯氨酸含量高（7.9%）。放大实验（起始量为45千克）表明产率略低（18 - 25%）。乙酸提取后对鱼皮进行酶水解可使产率再提高23%（干重/干重），对预处理后的鱼皮进行酶水解产率可达60%（干重/干重）。
