Yeon Jae-Ho, Cheng Liang, He Quan-Quan, Kong Ling-Yi
State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, China; Amorepacific Shanghai R & I Center, Shanghai 201801, China.
State Key Laboratory of New Drug and Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai 200040, China.
Chin J Nat Med. 2014 Oct;12(10):782-5. doi: 10.1016/S1875-5364(14)60119-9. Epub 2014 Oct 31.
To study the chemical constituents of the roots and stem bark of Kadsura coccinea.
Compounds were isolated by column chromatography on silica gel and Sephadex LH-20, and finally purified by prep-HPLC. Their structures were elucidated by extensive spectroscopic methods, including 1D- and 2D-NMR, and HR-ESI-MS.
Two compounds were determined as (7'S,8'S,8R)-(8β,8'α)-dimethyl-4,4'-dihydroxy-5,3'-dimethoxy-5'-cyclolignan glucoside (1) and micrandiactone H (2), respectively.
Compunds 1 and 2 are new and neither showed inhibitory effects on nitric oxide (NO) production in lipopolysaccharide-induced RAW264.7 macrophages.
研究黑老虎根和茎皮的化学成分。
通过硅胶柱色谱和葡聚糖凝胶LH - 20分离化合物，最后用制备型高效液相色谱进行纯化。采用包括一维和二维核磁共振以及高分辨电喷雾电离质谱等多种光谱方法对其结构进行解析。
确定两种化合物分别为(7'S,8'S,8R)-(8β,8'α)-二甲基-4,4'-二羟基-5,3'-二甲氧基-5'-环木脂素葡萄糖苷(1)和小花五味子素H(2)。
化合物1和2为新化合物，且二者对脂多糖诱导的RAW264.7巨噬细胞中一氧化氮(NO)的产生均无抑制作用。
