Hahnke Sarah, Maus Irena, Wibberg Daniel, Tomazetto Geizecler, Pühler Alfred, Klocke Michael, Schlüter Andreas
Department of Bioengineering, Leibniz Institute for Agricultural Engineering Potsdam-Bornim (ATB), Max-Eyth-Allee 100, 14469 Potsdam, Germany.
Center for Biotechnology (CeBiTec), Institute for Genome Research and Systems Biology, Bielefeld University, Universitätsstr. 27, 33615 Bielefeld, Germany.
J Biotechnol. 2015 Jan 10;193:34-6. doi: 10.1016/j.jbiotec.2014.11.010. Epub 2014 Nov 20.
In this study, the whole genome sequence of the mesophilic, anaerobic Porphyromonadaceae bacterium strain ING2-E5B (LMG 28429, DSM 28696) is reported. The new isolate belongs to the phylum Bacteroidetes and was obtained from a biogas-producing lab-scale completely stirred tank reactor (CSTR) optimized for anaerobic digestion of maize silage in co-fermentation with pig and cattle manure. The genome of strain ING2-E5B contains numerous genes encoding proteins and enzymes involved in the degradation of complex carbohydrates and proteinaceous compounds. Moreover, it possesses genes catalyzing the production of volatile fatty acids. Hence, this bacterium was predicted to be involved in hydrolysis and acidogenesis during anaerobic digestion and biomethanation.
在本研究中，报道了嗜温厌氧卟啉单胞菌科细菌菌株ING2-E5B（LMG 28429，DSM 28696）的全基因组序列。该新分离株属于拟杆菌门，是从一个为玉米青贮与猪粪和牛粪共发酵厌氧消化而优化的实验室规模全混式搅拌槽反应器（CSTR）中获得的。ING2-E5B菌株的基因组包含许多编码参与复杂碳水化合物和蛋白质化合物降解的蛋白质和酶的基因。此外，它拥有催化挥发性脂肪酸产生的基因。因此，预计该细菌在厌氧消化和生物甲烷化过程中参与水解和产酸过程。
