Hassa Julia, Tubbesing Tom Jonas, Maus Irena, Heyer Robert, Benndorf Dirk, Effenberger Mathias, Henke Christian, Osterholz Benedikt, Beckstette Michael, Pühler Alfred, Sczyrba Alexander, Schlüter Andreas
Genome Research of Industrial Microorganisms, Center for Biotechnology (CeBiTec), Bielefeld University, Universitätsstrasse 27, 33615 Bielefeld, Germany.
Computational Metagenomics Group, Center for Biotechnology (CeBiTec), Bielefeld University, Universitätsstraße 27, 33615 Bielefeld, Germany.
Microorganisms. 2023 Sep 27;11(10):2412. doi: 10.3390/microorganisms11102412.
The current focus on renewable energy in global policy highlights the importance of methane production from biomass through anaerobic digestion (AD). To improve biomass digestion while ensuring overall process stability, microbiome-based management strategies become more important. In this study, metagenomes and metaproteomes were used for metagenomically assembled genome (MAG)-centric analyses to investigate a full-scale biogas plant consisting of three differentially operated digesters. Microbial communities were analyzed regarding their taxonomic composition, functional potential, as well as functions expressed on the proteome level. Different abundances of genes and enzymes related to the biogas process could be mostly attributed to different process parameters. Individual MAGs exhibiting different abundances in the digesters were studied in detail, and their roles in the hydrolysis, acidogenesis and acetogenesis steps of anaerobic digestion could be assigned. was an active hydrogenotrophic methanogen in all three digesters, whereas was more prevalent at higher process temperatures. Further analysis focused on MAGs, which were abundant in all digesters, indicating their potential to ensure biogas process stability. The most prevalent MAG belonged to the class ; this MAG was ubiquitous in all three digesters and exhibited activity in numerous pathways related to different steps of AD.
当前全球政策对可再生能源的关注凸显了通过厌氧消化（AD）从生物质中生产甲烷的重要性。为了在确保整个过程稳定性的同时改善生物质消化，基于微生物群落的管理策略变得更加重要。在本研究中，利用宏基因组和宏蛋白质组进行以宏基因组组装基因组（MAG）为中心的分析，以研究一个由三个不同运行的消化器组成的全规模沼气厂。分析了微生物群落的分类组成、功能潜力以及蛋白质组水平上表达的功能。与沼气过程相关的不同基因和酶丰度大多可归因于不同的过程参数。详细研究了在消化器中表现出不同丰度的单个MAG，并确定了它们在厌氧消化的水解、产酸和产乙酸步骤中的作用。在所有三个消化器中都是活跃的氢营养型产甲烷菌，而在较高的过程温度下更为普遍。进一步的分析集中在所有消化器中都丰富的MAG上，表明它们具有确保沼气过程稳定性的潜力。最普遍的MAG属于 纲；这个MAG在所有三个消化器中都普遍存在，并在与AD不同步骤相关的众多途径中表现出活性。
