Raj P, Bhan M K, Prasad A K, Kumar R, Bhandari N, Jayashree S
Indian J Med Res. 1989 Mar;89:65-8.
A rural cohort of 507 children, less than 3 yr of age was kept under weekly surveillance for 12 months, for morbidity due to diarrhoea. Stool specimens for rotavirus detection were obtained in 346 of the 360 diarrheal episodes occurring in 354 children and in 211 children without diarrhoeal symptoms. Rotavirus was detected by an ELISA test in 4 per cent of children with diarrhoea and 6.6 per cent of control subjects. Viral genome RNA was extracted by phenol chloroform method from all the positive samples and analysed by polyacrylamide gel electrophoresis followed by silver staining. All the strains isolated, exhibited the same electropherotype with a 'long' RNA migration pattern. This result contrasts with the genomic variability amongst rotavirus strains usually observed in different settings.
对一组507名3岁以下的农村儿童进行了为期12个月的每周监测，以了解腹泻发病率。在354名出现腹泻的儿童中的360次腹泻发作以及211名无腹泻症状的儿童中采集了用于检测轮状病毒的粪便标本。通过酶联免疫吸附测定试验在4%的腹泻儿童和6.6%的对照受试者中检测到轮状病毒。从所有阳性样本中通过苯酚氯仿法提取病毒基因组RNA，并通过聚丙烯酰胺凝胶电泳随后进行银染分析。所有分离出的毒株均表现出相同的电泳图谱，具有“长”RNA迁移模式。这一结果与通常在不同环境中观察到的轮状病毒株之间的基因组变异性形成对比。
