巴西马塞约人轮状病毒基因组的电泳研究。

Electrophoretic study of the genome of human rotavirus from Maceió, Brazil.

作者信息

Houly C A, Uchoa M M, Zaidan A M, Gomes-Neto A, de-Oliveira F M, Athayde M A, Almeida M F, Pereira H G

出版信息

Braz J Med Biol Res. 1986;19(1):33-7.

Abstract

Rotaviruses were detected by enzyme immunoassay (EIA) in 53 (13.3%) of 397 fecal samples from children with acute gastroenteritis in the city of Maceió, Alagoas, Brazil. Polyacrylamide gel electrophoretic (PAGE) patterns characteristic of rotavirus double-stranded RNA were detected in 51 (96.2%) of the 53 EIA-positive samples. Of the RNA-positive samples, 1 (2%) was classified as subgroup 1 (short profile), 49 (96%) as subgroup 2 (long profile) and 1 (2%) could not be classified because of the absence of bands 10 and 11. The strains of subgroup 2 showed a great degree of electrophoretic heterogeneity and could be divided into several subcategories. Two samples showed splitting of one of the genome segments. PAGE, a very sensitive method capable of identifying rotavirus RNA genomes, has demonstrated that human rotaviruses detected in Maceió present many differences in RNA electrophoretic patterns.

摘要

在巴西阿拉戈斯州马塞约市，对397名急性肠胃炎患儿的粪便样本进行酶免疫测定（EIA），检测出53份（13.3%）样本含有轮状病毒。在53份EIA呈阳性的样本中，51份（96.2%）检测出具有轮状病毒双链RNA特征的聚丙烯酰胺凝胶电泳（PAGE）图谱。在RNA呈阳性的样本中，1份（2%）被归类为1型亚组（短图谱），49份（96%）为2型亚组（长图谱），1份（2%）因缺乏第10和11条带而无法分类。2型亚组的毒株表现出高度的电泳异质性，可分为几个亚类。两份样本显示其中一个基因组片段出现分裂。PAGE是一种能够识别轮状病毒RNA基因组的非常灵敏的方法，它表明在马塞约检测到的人类轮状病毒在RNA电泳图谱上存在许多差异。