Brambilla Bagatini Pamela, Xavier Léder Leal, Neves LauraTartari, Saur Lisiani, Barbosa Sílvia, Baptista Pedro Porto Alegre, Augustin Otávio Américo, Nunes de Senna Priscylla, Mestriner Régis Gemerasca, Souto André Arigony, Achaval Matilde
Brain Res. 2014 Dec 10;1592:101-12. doi: 10.1016/j.brainres.2014.10.007.
This study evaluated the effects of resveratrol on locomotor behaviors, neuronal and glial densities, and tyrosine hydroxylase immunoreactivity in the substantia nigra pars compacta of rats with streptozotocin-induced diabetes. Animals were divided into four groups: non-diabetic rats treated with saline (SAL), non-diabetic rats treated with resveratrol (RSV), diabetic rats treated with saline (DM) and diabetic rats treated with resveratrol (DM+RSV). The animals received oral gavage with resveratrol (20 mg/kg) for 35 days. The open field test and the bar test were performed to evaluate bradykinesia and akinesia, respectively. The Nissl-stained neuronal and glial densities and the dopaminergic neuronal density were estimated using planar morphometry. Tyrosine hydroxylase immunoreactivity was evaluated using regional and cellular optical densitometry. In relation to the locomotor behaviors, it was observed that the DM group developed akinesia, which was attenuated by resveratrol in the DM+RSV group, while the DM and DM+RSV groups showed bradykinesia. Our main morpho-physiological results demonstrated: a decrease in the cellular tyrosine hydroxylase immunoreactivity in the DM group, which was attenuated by resveratrol in the DM+RSV group; a higher neuronal density in the RSV group, when compared to the DM and DM+RSV groups; an increase in the glial density in the DM group, which was also reversed by resveratrol in the DM+RSV group. Resveratrol treatment prevents akinesia development and restores neuronal tyrosine hydroxylase immunoreactivity and glial density in the substantia nigra pars compacta of diabetic rats, suggesting that this polyphenol could be a potential therapeutic option against diabetes-induced nigrostriatal dysfunctions.
本研究评估了白藜芦醇对链脲佐菌素诱导的糖尿病大鼠黑质致密部运动行为、神经元和神经胶质细胞密度以及酪氨酸羟化酶免疫反应性的影响。动物被分为四组：用生理盐水处理的非糖尿病大鼠（SAL）、用白藜芦醇处理的非糖尿病大鼠（RSV）、用生理盐水处理的糖尿病大鼠（DM）和用白藜芦醇处理的糖尿病大鼠（DM+RSV）。动物接受白藜芦醇（20mg/kg）灌胃35天。分别进行旷场试验和杆状试验以评估运动迟缓及运动不能。使用平面形态测量法估计尼氏染色的神经元和神经胶质细胞密度以及多巴胺能神经元密度。使用区域和细胞光密度测定法评估酪氨酸羟化酶免疫反应性。关于运动行为，观察到DM组出现运动不能，而白藜芦醇在DM+RSV组中使其减轻，同时DM组和DM+RSV组表现出运动迟缓。我们主要的形态生理学结果表明：DM组细胞酪氨酸羟化酶免疫反应性降低，而白藜芦醇在DM+RSV组中使其减轻；与DM组和DM+RSV组相比，RSV组神经元密度更高；DM组神经胶质细胞密度增加，白藜芦醇在DM+RSV组中也使其逆转。白藜芦醇治疗可预防糖尿病大鼠黑质致密部运动不能的发生，并恢复神经元酪氨酸羟化酶免疫反应性和神经胶质细胞密度，表明这种多酚可能是对抗糖尿病诱导的黑质纹状体功能障碍的潜在治疗选择。
