Kondath Sindhu, Srinivas Raghavan Bhuvanasree, Anantanarayanan Rajaram, Rajaram Rama
Biochemistry Laboratory, Central Leather Research Institute, Adyar, Chennai, India.
Bio-Physics Laboratory, Central Leather Research Institute, Adyar, Chennai, India.
Chem Biol Interact. 2014 Dec 5;224:78-88. doi: 10.1016/j.cbi.2014.09.025. Epub 2014 Oct 18.
There is significant interest in investigating the therapeutic potential of phytochemical reduced and bound gold nanoparticles (AuNPs) as it bridges the gap between nanotechnology and therapy. In the present study, AuNPs prepared using the flavonoid morin (mAuNPs) are characterised and have been studied for their anti-cancer effects. The -OH groups of morin reduce Au(3+) and stabilize Au(0) to form spherical and crystalline mAuNPs. These mAuNPs are biocompatible towards normal human blood cells and breast epithelial cells. Through TEM analysis, we report that they are readily taken up by breast cancer cells (MCF-7) to induce cell death. Apoptosis has also been assessed by other morphological observations and cell viability studies. Flow cytometric studies reveal that the cells undergo a transient phase of apoptosis progressing towards secondary necrosis as the dose and time of mAuNPs treatment increases. The ability of mAuNPs to induce cell death in MCF-7 cells indicates its potential as an anti-cancer agent.
研究植物化学还原和结合的金纳米颗粒（AuNPs）的治疗潜力具有重大意义，因为它填补了纳米技术与治疗之间的空白。在本研究中，使用类黄酮桑色素制备的金纳米颗粒（mAuNPs）进行了表征，并对其抗癌作用进行了研究。桑色素的-OH基团还原Au(3+)并稳定Au(0)，形成球形和结晶的mAuNPs。这些mAuNPs对正常人血细胞和乳腺上皮细胞具有生物相容性。通过透射电子显微镜（TEM）分析，我们报告它们很容易被乳腺癌细胞（MCF-7）摄取以诱导细胞死亡。还通过其他形态学观察和细胞活力研究评估了细胞凋亡。流式细胞术研究表明，随着mAuNPs处理的剂量和时间增加，细胞经历一个短暂的凋亡阶段，进而发展为继发性坏死。mAuNPs在MCF-7细胞中诱导细胞死亡的能力表明其作为抗癌剂的潜力。
