Ponrasu Thangavel, Veerasubramanian Praveen Krishna, Kannan Ramya, Gopika Selvakumar, Suguna Lonchin, Muthuvijayan Vignesh
Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras Chennai 600036 India
Department of Chemistry, Indian Institute of Technology Madras Chennai 600036 India.
RSC Adv. 2018 Jan 9;8(5):2305-2314. doi: 10.1039/c7ra10334d.
Chronic wounds cost several billion dollars of public healthcare spending annually and continue to be a persistent threat globally. Several treatment methods have been explored, and all of them involve covering up the wound with therapeutic dressings that reduce inflammation and accelerate the healing process. In this present study, morin (MOR) was loaded onto hydrogel scaffolds prepared from psyllium seed husk polysaccharide (PSH), and human hair keratins (KER) crosslinked with sodium trimetaphosphate. ATR-FTIR confirmed the presence of the constituent chemical ingredients. SEM images of the scaffold surface reveal a highly porous architecture, with about 80% porosity measured by liquid displacement measurement, irrespective of the morin concentration. Swelling assays carried out on the scaffolds portray an ability to absorb up to seven times their dry weight of fluids. This makes them attractive for guiding moist wound healing on medium exuding wounds. An Alamar blue assay of NIH/3T3 fibroblast cells shows that cell viability decreases in the first 24 h but recovers to 85% in comparison to a control after 48 h. SEM images of fibroblast cells grown on the scaffolds confirm cellular attachment. An diabetic wound healing study showed that PSH + KER + MOR scaffold treatment significantly reduced the re-epithelialization time ( < 0.01) and enhanced the rate of wound contraction ( < 0.001), by accelerating collagen synthesis in diabetic rats compared to controls.
慢性伤口每年耗费数十亿美元的公共医疗支出，并且在全球范围内仍然是一个持续存在的威胁。人们已经探索了几种治疗方法，所有这些方法都涉及用能减轻炎症并加速愈合过程的治疗性敷料覆盖伤口。在本研究中，将桑色素（MOR）负载到由车前草籽壳多糖（PSH）制备的水凝胶支架上，并用三聚偏磷酸钠交联人发角蛋白（KER）。衰减全反射傅里叶变换红外光谱（ATR-FTIR）证实了组成化学成分的存在。支架表面的扫描电子显微镜（SEM）图像显示出高度多孔的结构，通过液体置换测量法测得孔隙率约为80%，与桑色素浓度无关。对支架进行的溶胀试验表明其具有吸收高达自身干重七倍液体的能力。这使得它们在引导中度渗出性伤口的湿性愈合方面具有吸引力。对NIH/3T3成纤维细胞进行的alamar蓝试验表明，细胞活力在最初24小时内下降，但48小时后与对照组相比恢复到85%。在支架上生长的成纤维细胞的SEM图像证实了细胞附着。一项糖尿病伤口愈合研究表明，与对照组相比，PSH + KER + MOR支架治疗通过加速糖尿病大鼠的胶原蛋白合成，显著缩短了再上皮化时间（<0.01）并提高了伤口收缩率（<0.001）。
