MutSα和MutLα免疫表达分析在口腔上皮发育异常和鳞状细胞癌诊断分级中的应用
MutSα and MutLα immunoexpression analysis in diagnostic grading of oral epithelial dysplasia and squamous cell carcinoma.
作者信息
Jessri M, Dalley A J, Farah C S
机构信息
The University of Queensland, UQ Centre for Clinical Research, Herston, QLD 4029, Australia; The University of Queensland, School of Dentistry, Brisbane, QLD 4000, Australia.
The University of Queensland, UQ Centre for Clinical Research, Herston, QLD 4029, Australia.
出版信息
Oral Surg Oral Med Oral Pathol Oral Radiol. 2015 Jan;119(1):74-82. doi: 10.1016/j.oooo.2014.06.017. Epub 2014 Sep 28.
OBJECTIVES
This study explored the expression of DNA mismatch repair (MMR) proteins in a range of oral biopsies. We further evaluated the significance of MMR protein expression combined with basic demographic data in differentiating grades of oral epithelial dysplasia (OED) and oral squamous cell carcinoma (OSCC).
STUDY DESIGN
Immunohistochemical expression of MutSα (hMLH1 and hPMS2) and MutLα (hMSH2 and hMSH6) were compared in 98 formalin-fixed paraffin embedded oral biopsies: 21 normal, 24 mild-dysplasia (MD), 8 moderate-to-severe-dysplasia (SD), and 45 OSCC.
RESULTS
Expression of hMLH1, hPMS2, and hMSH2 was reduced in MD, SD, and OSCC compared with the normal. Reduced hMSH2 immunoreactivity discriminated poorly differentiated OSCC from well-differentiated OSCC. The diagnostic model correctly classified 71.4% of cases and revealed that hPMS2-negative biopsies were more likely to be cancerous (odds ratio [OR], 0.11; 95% confidence interval [CI], 0.000-0.813; P = .040).
CONCLUSION
The results suggested a diagnostic role for MMR proteins in OED and OSCC.
目的
本研究探讨了一系列口腔活检组织中DNA错配修复（MMR）蛋白的表达情况。我们进一步评估了MMR蛋白表达结合基本人口统计学数据在区分口腔上皮发育异常（OED）等级和口腔鳞状细胞癌（OSCC）中的意义。
研究设计
比较了98例福尔马林固定石蜡包埋的口腔活检组织中MutSα（hMLH1和hPMS2）和MutLα（hMSH2和hMSH6）的免疫组化表达情况，其中包括21例正常组织、24例轻度发育异常（MD）、8例中度至重度发育异常（SD）和45例OSCC。
结果
与正常组织相比，MD、SD和OSCC中hMLH1、hPMS2和hMSH2的表达降低。hMSH2免疫反应性降低难以区分低分化OSCC和高分化OSCC。诊断模型正确分类了71.4%的病例，并显示hPMS2阴性活检组织更有可能是癌性的（优势比[OR]，0.11；95%置信区间[CI]，0.000 - 0.813；P = .040）。
结论
结果表明MMR蛋白在OED和OSCC中具有诊断作用。
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