Biodiversity Institute of Ontario, University of Guelph, 50 Stone Road East, Guelph, Canada N1G 2W1.
Biodiversity Institute of Ontario, University of Guelph, 50 Stone Road East, Guelph, Canada N1G 2W1.
Trends Parasitol. 2014 Dec;30(12):582-91. doi: 10.1016/j.pt.2014.09.003. Epub 2014 Oct 21.
For over 10 years, DNA barcoding has been used to identify specimens and discern species. Its potential benefits in parasitology were recognized early, but its utility and uptake remain unclear. Here we review studies using DNA barcoding in parasites and vectors affecting humans and find that the technique is accurate (accords with author identifications based on morphology or other markers) in 94-95% of cases, although aspects of DNA barcoding (vouchering, marker implicated) have often been misunderstood. In a newly compiled checklist of parasites, vectors, and hazards, barcodes are available for 43% of all 1403 species and for more than half of 429 species of greater medical importance. This is encouraging coverage that would improve with an active campaign targeting parasites and vectors.
十多年来，DNA 条码技术一直被用于鉴定样本和区分物种。其在寄生虫学中的潜在益处很早就被认识到，但它的实用性和采用率仍不清楚。在这里，我们回顾了使用 DNA 条码技术鉴定影响人类的寄生虫和病媒的研究，发现该技术在 94-95%的情况下是准确的（与基于形态或其他标记的作者鉴定相符），尽管 DNA 条码技术的某些方面（凭证、标记涉及）经常被误解。在一份新汇编的寄生虫、病媒和危害清单中，条码可用于 1403 种全部物种中的 43%，以及 429 种更具医学重要性物种中的一半以上。这是令人鼓舞的覆盖率，如果针对寄生虫和病媒开展积极的活动，覆盖率将会提高。
