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一种用于3D细胞扩增和重构的热响应性磁性胶体。

A thermoresponsive and magnetic colloid for 3D cell expansion and reconfiguration.

作者信息

Saeed Aram, Francini Nora, White Lisa, Dixon James, Gould Toby, Rashidi Hassan, Al Ghanami Racha Cheikh, Hruschka Veronika, Redl Heinz, Saunders Brian R, Alexander Cameron, Shakesheff Kevin M

机构信息

School of Pharmacy, University of East Anglia, Norwich, NR4 7TJ, UK.

出版信息

Adv Mater. 2015 Jan 27;27(4):662-8. doi: 10.1002/adma.201403626. Epub 2014 Nov 29.

DOI:10.1002/adma.201403626
PMID:25447597
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4322481/
Abstract

A dual thermoresponsive and magnetic colloidal gel matrix is described for enhanced stem-cell culture. The combined properties of the material allow enzyme-free passaging and expansion of mesenchymal stem cells, as well as isolation of cells postculture by the simple process of lowering the temperature and applying an external magnetic field. The colloidal gel can be reconfigured with thermal and magnetic stimuli to allow patterning of cells in discrete zones and to control movement of cells within the porous matrix during culture.

摘要

本文描述了一种用于增强干细胞培养的双热敏和磁性胶体凝胶基质。该材料的综合特性允许间充质干细胞在无酶的情况下传代和扩增，以及在培养后通过简单的降温及施加外部磁场的过程分离细胞。这种胶体凝胶可通过热和磁刺激重新配置，以实现细胞在离散区域的图案化，并在培养过程中控制细胞在多孔基质内的移动。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c294/4322481/eeb9e99962e7/adma0027-0662-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c294/4322481/be1e95dde2d8/adma0027-0662-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c294/4322481/3878fcb08b26/adma0027-0662-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c294/4322481/366ccbf183ee/adma0027-0662-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c294/4322481/15b1863e661d/adma0027-0662-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c294/4322481/eeb9e99962e7/adma0027-0662-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c294/4322481/be1e95dde2d8/adma0027-0662-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c294/4322481/3878fcb08b26/adma0027-0662-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c294/4322481/366ccbf183ee/adma0027-0662-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c294/4322481/15b1863e661d/adma0027-0662-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c294/4322481/eeb9e99962e7/adma0027-0662-f5.jpg

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Proteomic comparison of 3D and 2D glioma models reveals increased HLA-E expression in 3D models is associated with resistance to NK cell-mediated cytotoxicity.3D和2D胶质瘤模型的蛋白质组学比较显示，3D模型中HLA-E表达增加与对自然杀伤细胞介导的细胞毒性的抗性相关。
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Non-enzymatic dissociation of human mesenchymal stromal cells improves chemokine-dependent migration and maintains immunosuppressive function.
通过牺牲性糖蛋白复合材料的增材制造构建具有图案化血管网络的人体尺度组织。
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Mucin-Inspired Thermoresponsive Synthetic Hydrogels Induce Stasis in Human Pluripotent Stem Cells and Human Embryos.黏液启发的温度响应性合成水凝胶诱导人多能干细胞和人类胚胎停滞。
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非酶解分离人基质间充质干细胞可改善趋化因子依赖性迁移并保持免疫抑制功能。
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3D bioprinting of vascularized, heterogeneous cell-laden tissue constructs.三维生物打印血管化、异质细胞负载组织构建体。
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Mesenchymal stem cells for regenerative therapy: optimization of cell preparation protocols.用于再生治疗的间充质干细胞：细胞制备方案的优化
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