Uchida Takashi, Kadhum Wesam R, Kanai Sayumi, Todo Hiroaki, Oshizaka Takeshi, Sugibayashi Kenji
Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan.
Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan.
Eur J Pharm Sci. 2015 Jan 25;67:113-118. doi: 10.1016/j.ejps.2014.11.002. Epub 2014 Nov 14.
The usefulness of the synthetic membrane, Strat-M™ as an alternative to human and animal skins was evaluated by estimating the skin permeabilities of chemical compounds.
Thirteen chemical compounds with molecular weights (M.W.) of 152-289 and lipophilicities (log Ko/w) of -0.9 to 3.5 were selected. Strat-M™, excised human skin, or hairless rat skin was set in a Franz-type diffusion cell and a saturated solution of each chemical compound was applied to determine membrane permeation profiles. The obtained permeability coefficients (log P) were compared among these membranes.
Elevations were observed in log P for Strat-M™ with an increase in the log Ko/w of the applied compounds, and similar results were observed with the human and hairless rat skins. A correlation was obtained in log P values between Strat-M™ and human or hairless rat skin. Furthermore, the diffusion and partition parameters of chemicals in Strat-M™ were similar to those in the excised human and rat skins. These results suggest that Strat-M™ could be used as an alternative to animal or human skin in permeation studies.
通过评估化合物的皮肤渗透性，来评价合成膜Strat-M™作为人和动物皮肤替代品的有效性。
选择了13种分子量为152 - 289且亲脂性（log Ko/w）为 - 0.9至3.5的化合物。将Strat-M™、切除的人皮肤或无毛大鼠皮肤置于Franz型扩散池中，并施加每种化合物的饱和溶液以测定膜渗透曲线。比较了这些膜获得的渗透系数（log P）。
随着所施加化合物log Ko/w的增加，Strat-M™的log P升高，人皮肤和无毛大鼠皮肤也观察到类似结果。Strat-M™与人或无毛大鼠皮肤的log P值之间存在相关性。此外，Strat-M™中化学物质的扩散和分配参数与切除的人皮肤和大鼠皮肤中的相似。这些结果表明，Strat-M™可作为渗透研究中动物或人皮肤的替代品。
