Gamba C O, Campos L C, Negreiros-Lima G L, Maciel-Lima K, Sousa L P, Estrela-Lima A, Ferreira E, Cassali G D
Departamento de Patologia Geral, Instituto de Ciência Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, Minas Gerais 31270-901, Brazil.
Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, Minas Gerais 31270-901, Brazil.
Res Vet Sci. 2014 Dec;97(3):554-9. doi: 10.1016/j.rvsc.2014.09.016. Epub 2014 Oct 7.
ZEB1 and ZEB2 have been recently related to cancer prognosis. We investigated their expression and its association with clinicopathological parameters and overall survival in invasive micropapillary carcinoma (IMPC), which is a metastasising neoplasm of the canine mammary gland. Immunohistochemical evaluation showed nuclear and cytoplasmic staining for ZEB2 and nuclear staining for ZEB1. 'In situ' areas presented higher positivity for cytoplasmic ZEB2 than invasive areas of IMPC did (p = 0.03). ZEB1 positivity was associated with a low histological grade (p = 0.01). A shorter overall survival rate was observed in IMPCs that were positive for cytoplasmic ZEB2 (p = 0.04). Antibodies specificity in canine species was confirmed by western blot. Our results indicated that cytoplasmic ZEB2 appears to be an important factor in the early stages of malignancy and predicts a poor overall survival rate for IMPC in this canine mammary cancer model. ZEB1 downregulation appears to be associated with the dedifferentiation process of IMPC.
ZEB1和ZEB2最近被认为与癌症预后相关。我们研究了它们在侵袭性微乳头状癌（IMPC）中的表达及其与临床病理参数和总生存期的关系，IMPC是犬乳腺的一种转移性肿瘤。免疫组织化学评估显示ZEB2呈核染色和胞质染色，ZEB1呈核染色。“原位”区域的胞质ZEB2阳性率高于IMPC的侵袭性区域（p = 0.03）。ZEB1阳性与低组织学分级相关（p = 0.01）。胞质ZEB2阳性的IMPC患者总生存率较低（p = 0.04）。通过蛋白质印迹法证实了抗体在犬类中的特异性。我们的结果表明，胞质ZEB2似乎是恶性肿瘤早期的一个重要因素，并预测在这种犬乳腺癌模型中IMPC的总生存率较差。ZEB1下调似乎与IMPC的去分化过程有关。
