Rodrigues Michele A, Gamba Conrado O, Faria Jerusa Araújo Quintão Arantes, Ferreira Ênio, Goes Alfredo M, Gomes Dawidson A, Cassali Geovanni D
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte CEP: 31270-901, MG, Brazil; Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte CEP: 31270-901, MG, Brazil.
Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte CEP: 31270-901, MG, Brazil.
Pathol Res Pract. 2016 Apr;212(4):340-4. doi: 10.1016/j.prp.2015.11.017. Epub 2015 Dec 4.
The epidermal growth factor receptor (EGFR) has been described in the nucleus of primary tumors. Accumulation of EGFR at the nucleus is linked to DNA synthesis and cell proliferation, but the pathological significance of nuclear EGFR is not completely understood. The aim of this study was to investigate the nuclear localization of EGFR in invasive micropapillary carcinoma (IMPC) that is an aggressive neoplasm of canine mammary gland. Confocal immunofluorescence of formalin and paraffin-embedded tissue was used to access the subcellular localization of EGFR. Our results demonstrated that EGFR co-localizes with the inner nuclear envelope marker, Lamin B1 in IMPC. Furthermore, EGFR was not localized within the nucleus or at the inner nuclear envelope membrane in mammary carcinoma in mixed tumor (CMT) that is associated with a better prognosis than other malignant histological types. This finding could be useful as a predictive biomarker of therapeutic response for IMPC.
表皮生长因子受体（EGFR）已在原发性肿瘤细胞核中被发现。EGFR在细胞核中的积累与DNA合成和细胞增殖有关，但核EGFR的病理意义尚未完全明确。本研究旨在调查EGFR在侵袭性微乳头状癌（IMPC）中的核定位，IMPC是犬乳腺的一种侵袭性肿瘤。采用福尔马林固定石蜡包埋组织的共聚焦免疫荧光法来确定EGFR的亚细胞定位。我们的结果表明，在IMPC中EGFR与内核膜标记物核纤层蛋白B1共定位。此外，在混合瘤性乳腺癌（CMT）中，EGFR不在细胞核内或内核膜上定位，CMT的预后比其他恶性组织学类型更好。这一发现可能作为IMPC治疗反应的预测生物标志物。
