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基于单克隆抗体的竞争性酶联免疫吸附测定法检测畜禽血清中O:4沙门氏菌抗体

Monoclonal antibody-based competitive enzyme-linked immunosorbent assay to detect antibodies to O:4 Salmonella in the sera of livestock and poultry.

作者信息

Aribam Swarmistha Devi, Ogawa Yohsuke, Matsui Hidenori, Hirota Jiro, Okamura Masashi, Akiba Masato, Shimoji Yoshihiro, Eguchi Masahiro

机构信息

Bacterial and Parasitic Disease Research Division, National Institute of Animal Health, NARO, Tsukuba, Ibaraki 305-0856, Japan.

Kitasato Institute for Life Sciences and Graduate School of Control Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.

出版信息

J Microbiol Methods. 2015 Jan;108:1-3. doi: 10.1016/j.mimet.2014.11.002. Epub 2014 Nov 13.

DOI:10.1016/j.mimet.2014.11.002
PMID:25447889
Abstract

Serotyping is an important element for surveillance of Salmonella. In this study, an anti-O:4 Salmonella monoclonal antibody-based competitive enzyme-linked immunosorbent assay that could identify Salmonella infection in cow, pig, horse, and chicken was developed. This detection system can therefore be useful for a wide range of animals and for humans.

摘要

血清分型是沙门氏菌监测的重要环节。在本研究中,开发了一种基于抗O:4沙门氏菌单克隆抗体的竞争性酶联免疫吸附测定法,可用于鉴定牛、猪、马和鸡中的沙门氏菌感染。因此,该检测系统对多种动物和人类都有用。

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