Tian Yi, Dong Qinglong, Ji Zhirui, Chi Fumei, Cong Peihua, Zhou Zongshan
Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, Liaoning 125100, PR China.
Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, Liaoning 125100, PR China.
Gene. 2015 Jan 25;555(2):277-90. doi: 10.1016/j.gene.2014.11.018. Epub 2014 Nov 12.
The MADS-box gene family is one of the most widely studied families in plants and has diverse developmental roles in flower pattern formation, gametophyte cell division and fruit differentiation. Although the genome-wide analysis of this family has been performed in some species, little is known regarding MADS-box genes in apple (Malus domestica). In this study, 146 MADS-box genes were identified in the apple genome and were phylogenetically clustered into six subgroups (MIKC(c), MIKC*, Mα, Mβ, Mγ and Mδ) with the MADS-box genes from Arabidopsis and rice. The predicted apple MADS-box genes were distributed across all 17 chromosomes at different densities. Additionally, the MADS-box domain, exon length, gene structure and motif compositions of the apple MADS-box genes were analysed. Moreover, the expression of all of the apple MADS-box genes was analysed in the root, stem, leaf, flower tissues and five stages of fruit development. All of the apple MADS-box genes, with the exception of some genes in each group, were expressed in at least one of the tissues tested, which indicates that the MADS-box genes are involved in various aspects of the physiological and developmental processes of the apple. To the best of our knowledge, this report describes the first genome-wide analysis of the apple MADS-box gene family, and the results should provide valuable information for understanding the classification, cloning and putative functions of this family.
MADS-box基因家族是植物中研究最为广泛的基因家族之一，在花型形成、配子体细胞分裂和果实分化等发育过程中发挥着多样的作用。尽管已在一些物种中对该家族进行了全基因组分析，但关于苹果（Malus domestica）中的MADS-box基因却知之甚少。在本研究中，在苹果基因组中鉴定出146个MADS-box基因，并与拟南芥和水稻的MADS-box基因一起在系统发育上聚类为六个亚组（MIKC(c)、MIKC*、Mα、Mβ、Mγ和Mδ）。预测的苹果MADS-box基因以不同密度分布在所有17条染色体上。此外，还分析了苹果MADS-box基因的MADS-box结构域、外显子长度、基因结构和基序组成。此外，还分析了所有苹果MADS-box基因在根、茎、叶、花组织以及果实发育的五个阶段中的表达情况。除每组中的一些基因外，所有苹果MADS-box基因都在至少一种测试组织中表达，这表明MADS-box基因参与了苹果生理和发育过程的各个方面。据我们所知，本报告描述了对苹果MADS-box基因家族的首次全基因组分析，其结果应为理解该家族的分类、克隆和假定功能提供有价值的信息。
