Wei Xin, Wang Linhai, Yu Jingyin, Zhang Yanxin, Li Donghua, Zhang Xiurong
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture, Wuhan 430062, China.
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture, Wuhan 430062, China.
Gene. 2015 Sep 10;569(1):66-76. doi: 10.1016/j.gene.2015.05.018. Epub 2015 May 9.
MADS-box genes encode transcription factors that play crucial roles in plant growth and development. Sesame (Sesamum indicum L.) is an oil crop that contributes to the daily oil and protein requirements of almost half of the world's population; therefore, a genome-wide analysis of the MADS-box gene family is needed. Fifty-seven MADS-box genes were identified from 14 linkage groups of the sesame genome. Analysis of phylogenetic relationships with Arabidopsis thaliana, Utricularia gibba and Solanum lycopersicum MADS-box genes was performed. Sesame MADS-box genes were clustered into four groups: 28 MIKC(c)-type, 5 MIKC(⁎)-type, 14 Mα-type and 10 Mγ-type. Gene structure analysis revealed from 1 to 22 exons of sesame MADS-box genes. The number of exons in type II MADS-box genes greatly exceeded the number in type I genes. Motif distribution analysis of sesame MADS-box genes also indicated that type II MADS-box genes contained more motifs than type I genes. These results suggested that type II sesame MADS-box genes had more complex structures. By analyzing expression profiles of MADS-box genes in seven sesame transcriptomes, we determined that MIKC(C)-type MADS-box genes played significant roles in sesame flower and seed development. Although most MADS-box genes in the same clade showed similar expression features, some gene functions were diversified from the orthologous Arabidopsis genes. This research will contribute to uncovering the role of MADS-box genes in sesame development.
MADS盒基因编码在植物生长发育中起关键作用的转录因子。芝麻（Sesamum indicum L.）是一种油料作物，满足了世界近一半人口日常的油脂和蛋白质需求；因此，需要对MADS盒基因家族进行全基因组分析。从芝麻基因组的14个连锁群中鉴定出57个MADS盒基因。对其与拟南芥、狸藻和番茄的MADS盒基因进行了系统发育关系分析。芝麻MADS盒基因被分为四组：28个MIKC(c)型、5个MIKC(⁎)型、14个Mα型和10个Mγ型。基因结构分析显示芝麻MADS盒基因的外显子数量为1至22个。II型MADS盒基因的外显子数量大大超过I型基因。芝麻MADS盒基因的基序分布分析也表明，II型MADS盒基因比I型基因含有更多的基序。这些结果表明，II型芝麻MADS盒基因具有更复杂的结构。通过分析七个芝麻转录组中MADS盒基因的表达谱，我们确定MIKC(C)型MADS盒基因在芝麻花和种子发育中起重要作用。虽然同一进化枝中的大多数MADS盒基因表现出相似的表达特征，但一些基因功能与拟南芥直系同源基因有所不同。这项研究将有助于揭示MADS盒基因在芝麻发育中的作用。
