Troczka B J, Williams A J, Bass C, Williamson M S, Field L M, Davies T G E
Biological Chemistry and Crop Protection Department, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK.
Institute of Molecular & Experimental Medicine, Cardiff University School of Medicine, Wales Heart Research Institute, Heath Park, Cardiff CF14 4XN, UK.
Gene. 2015 Feb 10;556(2):106-12. doi: 10.1016/j.gene.2014.11.035. Epub 2014 Nov 20.
The peach potato aphid, Myzus persicae, is one of the most important agricultural pests of temperate climates. It is mainly controlled through the judicious application of insecticides; however, over time, aphids have developed resistance to many insecticidal classes. The recent introduction of synthetic diamide insecticides, with a novel mode of action, potentially offers new tools to control aphid populations. These diamides act on the ryanodine receptor (RyR), a large endoplasmic calcium release channel. In this study we have cloned cDNAs encoding the complete open reading frame of the RyR from M. persicae. The open reading frame is 15,306 base pairs long and encodes a protein of 5101 amino acids. The aphid RyR shares many of the features of other insect and vertebrate RyRs, including a highly conserved transmembrane region. However, unlike the other RyRs characterised to date, the M. persicae channel does not display alternative splicing at any stage of its developmental cycle, so it cannot generate functional variants of the channel.
桃蚜（Myzus persicae）是温带气候下最重要的农业害虫之一。主要通过合理使用杀虫剂进行防治；然而，随着时间的推移，蚜虫已对许多种类的杀虫剂产生了抗性。最近引入的具有新型作用模式的合成双酰胺杀虫剂，可能为控制蚜虫种群提供新工具。这些双酰胺作用于兰尼碱受体（RyR），这是一种大型内质网钙释放通道。在本研究中，我们克隆了编码桃蚜RyR完整开放阅读框的cDNA。该开放阅读框长15306个碱基对，编码一个由5101个氨基酸组成的蛋白质。蚜虫RyR具有许多其他昆虫和脊椎动物RyR的特征，包括一个高度保守的跨膜区域。然而，与迄今为止已鉴定的其他RyR不同，桃蚜通道在其发育周期的任何阶段都不显示可变剪接，因此它不能产生通道的功能变体。
