Kostyleva E I, Selivanova G V, Zalenskaia I A
Mol Biol (Mosk). 1989 Jan-Feb;23(1):73-9.
A comparative analysis of chromatin from erythrocytes of frog, trout and hen has been performed in correlation with properties of the nucleosomal linker histones of H1 family. In the nucleosomes from frog erythrocytes the linker histone is represented by H1(0)-like variant with amino acid sequence highly homologous to that of the hen histone H5, however the arginine content in the proteins differs (3 mol% in the frog erythrocyte H1 and 12 mol% in the hen erythrocyte H5). On the other hand histone H5 from trout being significantly different in the primary structure from the hen histone H5 is at the same time rich in arginine (9 mol%). The nucleosomal repeat length, estimated by using agarose gel electrophoresis is 201, 213 and 213 b.p. in erythrocyte chromatin from frog, trout and hen, correspondingly. Chromatin packing density in fixed nuclei from erythrocytes of frog, trout and hen as determined using cytophotometric measurements is 0.144, 0.444 and 530 pg/mu 3, correspondingly. The data support the previously made suggestion that the increase in arginine content in nucleosomal linker proteins is connected with the increase of chromatin compaction in the nuclei and elongation of the linker in the nucleosome.
对青蛙、鳟鱼和母鸡红细胞中的染色质进行了比较分析，并与H1家族核小体连接组蛋白的特性相关联。在青蛙红细胞的核小体中，连接组蛋白由类似H1(0)的变体代表，其氨基酸序列与母鸡组蛋白H5高度同源，然而蛋白质中的精氨酸含量不同（青蛙红细胞H1中为3摩尔%，母鸡红细胞H5中为12摩尔%）。另一方面，鳟鱼的组蛋白H5在一级结构上与母鸡组蛋白H5有显著差异，同时富含精氨酸（9摩尔%）。通过琼脂糖凝胶电泳估计，青蛙、鳟鱼和母鸡红细胞染色质中的核小体重复长度分别为201、213和213碱基对。使用细胞光度测量法测定，青蛙、鳟鱼和母鸡红细胞固定核中的染色质堆积密度分别为0.144、0.444和530皮克/立方微米。这些数据支持了之前提出的观点，即核小体连接蛋白中精氨酸含量的增加与细胞核中染色质压缩的增加以及核小体中连接体的延长有关。
