组蛋白H1和H5含量不同的红细胞染色质的DNA重复长度

DNA repeat lengths of erythrocyte chromatins differing in content of histones H1 and H5.

作者信息

Miki B L, Neelin J M

出版信息

Nucleic Acids Res. 1980 Feb 11;8(3):529-42. doi: 10.1093/nar/8.3.529.

DOI:10.1093/nar/8.3.529

PMID:6777761

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327287/

Abstract

Among the erythrocytes of chicken, trout, carp, and sucker, the relative proportion of the lysine-rich histone H5 varied from 20 to 0% of the total histones. Following digestion of nuclear chromatin with micrococcal nuclease, each of them displayed a longer DNA repeat length and greater repeat length heterogeneity than found in liver chromatin. Fish erythrocytes possessed similar repeat lengths of 207-209 base pairs which was 10-12 base pairs shorter than in chicken erythrocyte chromatin and approximately 10 base pairs longer than in liver chromatin. No correlation existed between the DNA repeat length or repeat length heterogeneity and the relative proportion of H5.

摘要

在鸡、鳟鱼、鲤鱼和吸盘鱼的红细胞中，富含赖氨酸的组蛋白H5在总组蛋白中的相对比例在20%至0%之间变化。用微球菌核酸酶消化核染色质后，它们每一个都显示出比肝脏染色质更长的DNA重复长度和更大的重复长度异质性。鱼类红细胞具有207 - 209个碱基对的相似重复长度，比鸡红细胞染色质短10 - 12个碱基对，比肝脏染色质长约10个碱基对。DNA重复长度或重复长度异质性与H5的相对比例之间不存在相关性。