Graduate Program in Developmental Biology, Baylor College of Medicine, Houston, TX 77030, USA; Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.
Dev Biol. 2015 Jan 15;397(2):203-11. doi: 10.1016/j.ydbio.2014.11.006. Epub 2014 Nov 20.
Extracellular cAMP functions as a primary ligand for cell surface cAMP receptors throughout Dictyostelium discoideum development, controlling chemotaxis and morphogenesis. The developmental consequences of cAMP signaling and the metabolism of cAMP have been studied in great detail, but it has been unclear how cells export cAMP across the plasma membrane. Here we show pharmacologically and genetically that ABC transporters mediate cAMP export. Using an evolutionary-developmental biology approach, we identified several candidate abc genes and characterized one of them, abcB3, in more detail. Genetic and biochemical evidence suggest that AbcB3 is a component of the cAMP export mechanism in D. discoideum development.
细胞外 cAMP 作为一种主要的配体，通过细胞表面的 cAMP 受体在整个盘基网柄菌发育过程中发挥作用，控制着趋化性和形态发生。cAMP 信号转导和 cAMP 的代谢的发育后果已经被深入研究，但细胞如何将 cAMP 穿过质膜输出仍不清楚。在这里，我们通过药理学和遗传学方法表明 ABC 转运蛋白介导 cAMP 输出。通过一种进化发育生物学的方法，我们鉴定了几个候选的 abc 基因，并进一步详细描述了其中一个基因，abcB3。遗传和生化证据表明，AbcB3 是 D. discoideum 发育中 cAMP 输出机制的一个组成部分。
